Extracellular matrix organization
New and Updated Topics and Pathways. Topics with new or revised pathways in this release include Cell Cycle (p53-Independent G1/S DNA Damage Checkpoint), Developmental Biology (Developmental Lineage of Mammary Gland Cells, Developmental Lineage of Mammary Gland Alveolar Cells, Developmental Lineage of Mammary Gland Luminal Epithelial Cells, Differentiation of naive CD4+ T cells to T helper 1 cells (Th1 cells)), Disease (Microbial factors inhibit CASP4 activity, SLC transport disorders), Immune System (Activation of C3 and C5, Non-canonical inflammasome activation, Regulation of PD-L1 transcription, Regulation of PD-L1 translation, Regulation of PD-L1 post-translation modification), Metabolism, Metabolism of proteins (Ribosome-associated quality control), and Transport of small molecules (SLC-mediated transmembrane transport).
New and Updated Illustrations. New or revised Illustrations with embedded navigation features have been created for Developmental biology, Developmental Lineages of the Mammary Gland, Differentiation of T cells, Extracellular matrix organization, Innate Immune System, Regulation of Expression and Function of Type II Classical Cadherins, SLC-mediated transmembrane transport, Translation, Drug resistance in ERBB2 KD mutants, and SLC transporter disorders.
Thanks to our Contributors. Mien-Chie Hung, Jingping Qiao, and Hirohito Yamaguchi are our external authors and Isabella Barbutti, Eric J Bennett, Huiquan Duan, Mien-Chie Hung, Pierce Ford, David P Hill, Graham M Lord, Jingping Qiao, Xuyan Shi and Hirohito Yamaguchiare our external reviewers.
Annotation Statistics. Reactome comprises 16,002 human reactions organized into 2,825 pathways involving 31,991 proteins and modified forms of proteins encoded by 11,410 different human genes, 15,751 complexes, 2,176 small molecules, and 1,070 drugs. These annotations are supported by 41,373 literature references. We have projected these reactions onto 80,701 orthologous proteins, creating 20,320 orthologous pathways in 14 non-human species. Version 94 has annotations for 5,507 protein variants (mutated proteins) and their post-translationally modified forms, derived from 392 proteins, which have contributed to the annotation of 1,888 disease-specific reactions and 763 pathways. Our illustration library now includes 2,500 icons and over 200 high-level interactive pathway Illustrations.
Other news:
As of Reactome V93, our Docker images are no longer available on DockerHub and have been migrated to AWS Elastic Container Registry (ECR). Users should pull images from AWS ECR moving forward. Updated access instructions are available in our documentation.
As of Reactome V94, the following files will no longer be available: "Protégé 2.0 ontology files", and "Events in the BioPAX level 2 format". If you require these files for your work, please let our team know via This email address is being protected from spambots. You need JavaScript enabled to view it..
Tools and Data. Our services and software tools are designed for biologists, bioinformaticians, and software developers. Pathway data is available to view in our Pathway Browser, to analyze your own dataset, to download, and access programmatically through our Content and Analysis Services. The Reactome FIViz app and ReactomeGSA package provide tools for multi-omics data analysis. The idg.reactome.org Web Portal provides a collection of web-based tools to help researchers place understudied proteins in a pathway context.
Documentation and Training. Visit our online User Guide to access documentation supporting pathway analysis of experimental data. The Developer's Zone provides detailed documentation regarding our software, tools, and web services. Training and learning materials can be found here.
About the Reactome Project. Reactome is a collaboration between groups at the Ontario Institute for Cancer Research, Oregon Health and Science University, New York University Langone Medical Center, and The EMBL - European Bioinformatics Institute. Reactome is both an ELIXIR Core Data Resource and a Global Core Biodata Resource and has been certified as a Trustworthy Data Repository by the CoreTrustSeal Standards and Certification Board.
Reactome annotation files and interaction data derived from Reactome are distributed under a Creative Commons Public Domain (CC0 1.0 Universal) Licence. A Creative Commons Attribution 4.0 International (CC BY 4.0) License applies to all software and code, database data dumps, and Pathway Illustrations (Enhanced High-Level Diagrams), Icon Library, Art, and Branding Materials. A full description of the new and updated content is available on the Reactome website.
Follow us on Twitter: @reactome to get frequent updates about new and updated pathways, feature updates, and more!
For more information: If you have a question, want to provide feedback, or are interested in collaborating with us to annotate a topic, please contact us at This email address is being protected from spambots. You need JavaScript enabled to view it..