Strain number NIES-2169
Phylum Chlorophyta
Class Trebouxiophyceae
Scientific name Chlorella sorokiniana Shihira & R.W.Krauss
Synonym
Former name Chlorella pyrenoidosa Chick
Common name Green alga
Locality (Date of collection) Waller Creek on Univ. TX Campus, Austin, Texas, USA
Latitude / Longitude 30.259697 /
Habitat (Isolation source) Freshwater
History < IAM (2007) < Meyers, Jack
Isolator (Date of isolation) Sorokin, C.
Identified by Kessler, E.; CConfirmed by DNA sequencing at NIES
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition
(Preculture condition)
Medium: C (agar)
Temperature: 20 C
Light intensity: 8-15 µmol photons/m2/sec, L/D cycle: 10L:14D
Duration: 3 M
Gene information 18S rRNA ( AB080307 AB488573 ) , actin ( AB080311 )
Cell size (min - max) 5 - 8 μm
Organization Unicellular
Characteristics Starch production (Takeshita et al. 2014)
Other strain no. Other collection strain no. : IAM C-212; ATCC 22521; CAUP H1957; CCAP 211/8k; SAG 211-8K; UTEX 1230
Remarks Cryopreserved; Axenic
Movie
Related strain information
(Large number of orders and same genus or species)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2168
Chlorella sorokiniana Green alga
NIES-2170
Chlorella vulgaris Green alga Starch production (Hirokawa et al. 1982) ; Genome decoded strain (Wakasugi et al. 1997)
NIES-642
Chlorella vulgaris Green alga Freshwater (River water)
NIES-227
Chlorella vulgaris Green alga
NIES-2540
Chlorella variabilis Green alga (Paramecium bursaria F36) Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2173
Chlorella sorokiniana Green alga Starch production (Miyachi et al. 1986)
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2275
Scenedesmus coelastroides Green alga
NIES-432
Carteria radiosa Green alga Freshwater (Pond water)
NIES-2201
Chloromonas actinochloris Green alga Hydrogen evolution
NIES-1048
Chlamydomonas noctigama Green alga Freshwater Phototaxis
NIES-2179
Parachlorella kessleri Green alga Starch production (Takeshita et al. 2014)
Reference
Ohta, S., Miyamoto, K., Miura, Y. 1987 Hydrogen evolution as a consumption mode of reducing equivalents in green algal fermentation. Plant Physiol., 83, 1022-1026.
Strain(s): 2169, 3284
PubMed: 16665317
DOI: 10.1104/pp.83.4.1022

Maruyama, I., Ando, Y., Maeda, T., Hirayama, K. 1989 Uptake of vitamin B12 by various strains of unicellular algae Chlorella. Nippon Suisan Gakkaishi, 55, 1785-1790.
Strain(s): 48, 226, 687, 2157, 2169, 2175

Brand, J. J. & Wright, J. N. 1989 Hydrogen production by eukaryotic algae. Biotech. Bioeng., 33, 1482-1488.
Strain(s): 2151, 2169, 2190, 2194, 2201, 2202, 2203, 2204, 2211, 2213, 2219, 2279, 2291

Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 686, 687, 2150, 2151, 2160, 2163, 2169, 2175, 2176
DOI: 10.1111/j.0022-3646.1992.00550.x

Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184.
Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection
Strain(s): 2160, 2169, 2173, 2186, 4016
DOI: 10.1046/j.1529-8817.2003.02052.

Mizuno, Y., Sato, A., Watanabe, K., Hirata, A., Takeshita, T., Ota, S., Sato, N., Zachleder, V., Tsuzuki, M., Kawano, S. 2013 Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species. Bioresour. Technol., 129, 150-155.
Keywords: Sulfur deficiency; Chlorella spp.; Parachlorella kessleri; Starch; Lipids
Strain(s): 2169, 2173
PubMed: 23238344
DOI: 10.1016/j.biortech.2012年11月03日0

Imase, M., Ohko, Y., Takeuchi, M., Hanada, S. 2013 Estimating the viability of Chlorella exposed to oxidative stresses based around photocatalysis. Int. Biodeterior. Biodegrad., 78, 1-6.
Keywords: Autofluorescence; Cell staining; Chlorella sorokiniana; Oxidative stress: TiO2 photocatalysis; Viability assay
Strain(s): 2169
DOI: 10.1016/j.ibiod.201212006

Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134.
Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light
Strain(s): 2151, 2152, 2159, 2169, 2170, 2179
PubMed: 24583913
DOI: 10.1016/j.biortech.2014年01月13日5

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133.
Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS
Strain(s): 35, 144, 362, 418, 421, 432, 438, 456, 545, 571, 732, 737, 858, 1022, 1048, 1848, 2150, 2169, 2201, 2202, 2208, 2209, 2212, 2280, 2352, 2462, 2578
PubMed: 30304548
DOI: 10.1111/jpy.12798

Kato, T., Nakano, K., Sueyoshi, M., Tasaki, M., Kuroiwa, Y. 2020 Large-scale outdoor test of Chlorella culturing in raceway system in the Middle East. Shimizu Kensetsu Kenkyuhokoku, 97, 133-138 (in Japanese with English summary).
Strain(s): 2169

Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. 2004 Haemagglutinating and antibiotic activities of freshwater microalgae. World J. Microbiol. Biotechnol., 20, 817–825.
Keywords: Antibiotic activity; erythrocytes; freshwater microalgae; haemagglutination
Strain(s): 226, 227, 2159, 2163, 2165, 2169, 2175, 2189, 2208, 2211, 2213, 2214, 2229, 2231, 2233, 2240, 2241, 2243, 2247, 2248, 3277, 3284
DOI: 10.1007/s11274-004-8712-6

Anam, K., Rahman, D. Y., Hidhayati, N., Rachmayati, R., Susilaningsih, D., Agustini, N. W. S., Praharyawan, S., Susanti, H., Apriastini, M. 2021 Lipid accumulation on optimized condition through biomass production in green algae. IOP Conf. Ser.: Earth Environ. Sci., 762, 012075 (article ID).
Strain(s): 2152, 2169, 2170
DOI: 10.1088/1755-1315/762/1/012075

Maltsev, Y., Maltseva, K., Kulikovskiy, M., Maltseva, S. 2021 Influence of light conditions on microalgae growth and content of lipids, carotenoids, and fatty acid composition. Biology-Basel, 10, 1060 (article ID).
Keywords: carotenoids; fatty acids; light; lipids; microalgae; photoperiod
Strain(s): 2151, 2152, 2159, 2169, 2170
PubMed: 34681157
DOI: 10.3390/biology10101060

Hirayama, A., Sueyoshi, M. N., Nakano, T., Ota, Y., Kurita, H., Tasaki, M., Kuroiwa, Y., Kato, T., Serizawa, S., Kojima, K., Al-Maamari, R. S., Hasegawa, T., Thomas-Hall, S. R., Schenk, P. M. 2022 .Development of large-scale microalgae production in the Middle East. Bioresour. Technol., 343, 126036 (article ID).
Keywords: Chlorella sorokiniana; Large-scale microalgae production; Outdoor raceway ponds; Photobioreactor; Water temperature control
Strain(s): 2169
PubMed: 34626761
DOI: 10.1016/j.biortech.2021.126036

Tawfik, A., Niaz, H., Qadeer, K., Qyyum, M. A., Liu, J. J., Lee, M. 2022 Valorization of algal cells for biomass and bioenergy production from wastewater: Sustainable strategies, challenges, and techno-economic limitations. Renew. Sust. Energ. Rev., 157, 112024 (article ID).
Keywords: Xenobiotic compounds; Micro-pollutants; Enzyme activity; Biomass; Bioenergy; Algae-based wastewater treatment
Strain(s): 19, 23, 44, 226, 422, 437, 2169, 2220, 3284
DOI: 10.1016/j.rser.2021.112024

Ota, S., Yoshimura, K., Kosugi, C., Kawano, S. 2023 Taxonomic and physiological studies of Parachlorella kimitsuensis sp. nov. (Trebouxiophyceae), which shows high ammonium tolerance. Algal Res., 71, 103054 (article ID).
Keywords: Chlorella; Halophiles; High ammonium; Parachlorella, Parachlorella kimitsuensis sp. nov.; Remediation; Steelmaking wastewater treatment
Strain(s): 2152, 2169, 2273, 2275
DOI: 10.1016/j.algal.2023.103054

Kodama, Y. & Endoh, Y. 2024 Comparative analyses of the symbiotic associations of the host Paramecium bursaria with free-living and native symbiotic species of Chlorella. Curr. Microbiol., 81, 66 (article ID).
Strain(s): 2169
PubMed: 38231280
DOI: 10.1007/s00284-023-03590-9

Sweiss, M., Hasan, M., Odat, N. 2024 Develop strain-specific simple sequence repeat (SSR) markers for Chlorella sorokiniana. J. Microbiol. Biotechnol., 34, 1848-1856.
Keywords: Chlorella; simple sequence repeats; microsatellites; strain-specific
Strain(s): 2169
PubMed: 39187448
DOI: 10.4014/jmb.2404.04024

Kodama, Y., & Fujishima, M. 2024 Effects of the symbiotic Chlorella variabilis on the host ciliate Paramecium bursaria phenotypes. Microorganisms, 12, 2537 (article ID).
Keywords: Chlorella sp.; Chlorella variabilis; cytoplasmic crystal; digestive vacuole; endosymbiosis; heat tolerance; mitochondria; Paramecium bursaria; perialgal vacuole; photoaccumulation; trichocyst
Strain(s): 2169
PubMed: 39770740
DOI: 10.3390/microorganisms12122537

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