Strain number NIES-2540
Phylum Chlorophyta
Class Trebouxiophyceae
Scientific name Chlorella variabilis Shihira & Krauss
Synonym Chlorella paramecii Loefer 1936
Former name
Common name Green alga
Locality (Date of collection) Japan
Latitude / Longitude
Habitat (Isolation source) (Paramecium bursaria F36)
History < Hoshina, Ryo
Isolator (Date of isolation) Kamako, Shin-ichiro (2002-**-**)
Identified by Hoshina, Ryo
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2024 May]
Culture condition
(Preculture condition)
Medium: CYT ; HUT
Temperature: 20 C
Light intensity: 4-10 µmol photons/m2/sec, L/D cycle: 10L:14D
Duration: 1 M
Gene information 16S rRNA ( AB208588 ) , 18S-ITS1-5.8S-ITS2 ( AB162914 ) , rbcL ( AB260900 ) , psaA ( AB260914 )
Cell size (min - max) 5 - 8 μm
Organization Unicellular
Characteristics Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid)
Other strain no. Other strain no. : F36-ZK
Remarks Cryopreserved; Axenic
Movie
Related strain information
(Large number of orders and same genus or species)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2168
Chlorella sorokiniana Green alga
NIES-2169
Chlorella sorokiniana Green alga Freshwater Starch production (Takeshita et al. 2014)
NIES-2170
Chlorella vulgaris Green alga Starch production (Hirokawa et al. 1982) ; Genome decoded strain (Wakasugi et al. 1997)
NIES-642
Chlorella vulgaris Green alga Freshwater (River water)
NIES-227
Chlorella vulgaris Green alga
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2173
Chlorella sorokiniana Green alga Starch production (Miyachi et al. 1986)
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-3983
Yamagishiella unicocca Green alga Freshwater Phototaxis ; Genome decoded strain (Hamaji et al. 2018) ; backcross strain of NIES-1861 x F1 clone (NIES-1861 x 1859) ; Heterothallic ; Isogamous ; Mating type (−) ; Resting spore-forming ; MID gene (+)
NIES-2157
Parachlorella kessleri Green alga Starch production (Miyachi et al. 1986)
NIES-1271
Trebouxia anticipata Green alga Terrestrial (The lichen Punctelia rudecta on a stone column) Symbiotic (lichen)
NIES-2261
Gonium pectorale Green alga Freshwater (Paddy soil) Phototaxis ; Heterothallic ; Mating type (+)
NIES-242
Pandorina morum Green alga Freshwater (Lake water) Phototaxis ; Heterothallic ; Mating type (+) ; Crosses with NIES-243 and 362
Reference
Hoshina, R., Kamako, S.-I., Imamura, N. 2004 Phylogenetic position of endosymbiotic green algae in Paramecium bursaria Ehrenberg from Japan. Plant Biol., 6, 447-453.
Keywords: 18S rDNA; Chlorella; endosymbiont; ITS; Paramecium bursaria; phylogeny
Strain(s): 2540, 2541
DOI: 10.1055/s-2004-820888

Hoshina, R., Kato, Y., Kamako, S., Imamura, N. 2005 Genetic evidence of "American" and "European" type symbiotic algae of Paramecium bursaria Ehrenberg. Plant Biol., 7, 526-532.
Keywords: 18S rDNA; "American"; "European"; Chlorella; ITS; Paramecium bursaria; Phylogeny
Strain(s): 2540, 2541
DOI: 10.1055/s-2005-865912

Kato, Y., Ueno, S., Imamura, N. 2006 Studies on the nitrogen utilization of endosymbiotic algae isolated from Japanese Paramecium bursaria. Plant Sci., 170, 481-486.
Keywords: Endosymbiont; Paramecium bursaria; Nitrogen utilization; Chlorella
Strain(s): 227, 2540, 2541
DOI: 10.1016/j.plantsci.2005年09月01日8

Kato, Y., Imamura, N., 2008 Effect of calcium ion on uptake of amino acids by symbiotic Chlorella F36-ZK isolated from Japanese Paramecium bursaria. Plant Sci., 174, 88-96.
Keywords: Paramecium bursaria; Chlorella; Calcium; Divalent cation; Amino acid transport; Symbiosis
Strain(s): 227, 2157, 2540
DOI: 10.1016/j.plantsci.200710001

Quispe, C. F., Sonderman, O., Khasin, M., Riekhof, W. R., Van Etten, J. L., Nickerson, K. W. 2016 Comparative genomics, transcriptomics, and physiology distinguish symbiotic from free-living Chlorella strains. Algal Res., 18, 332-340.
Keywords: Chlorella variabilis; Alga-paramecium symbiosis; Algal nitrogen metabolism; Amino acid transporters
Strain(s): 2540, 2541
DOI: 10.1016/j.algal.201606001

Hoshina, R., Iwataki, M., Imamura, N. 2010 Chlorella variabilis and Micractinium reisseri sp. nov. (Chlorellaceae, Trebouxiophyceae): Redescription of the endosymbiotic green algae of Paramecium bursaria (Peniculia, Oligohymenophorea) in the 120th year. Phycol. Res., 58, 188-201.
Keywords: Chlorella variabilis; ITS2; Micractinium reisseri; Paramecium bursaria; species concept
Strain(s): 2540, 2542
DOI: 10.1111/j.1440-1835.2010.00579.x

Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82.
Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine
Strain(s): 18, 35, 242, 691, 735, 1271, 1278, 2185, 2187, 2235, 2261, 2540, 2572, 3983

Toyoshima, H., Miyata, A., Yoshida, R., Ishige, T., Takaichi, S., Kawasaki, S. 2021 Distribution of the water-soluble astaxanthin binding carotenoprotein (AstaP) in Scenedesmaceae. Mar. Drugs, 19, 349 (article ID).
Keywords: astaxanthin; Scenedesmus; Coelastrella; carotenoprotein; astaxanthin-binding protein
Strain(s): 2238, 2540
PubMed: 34203079
DOI: 10.3390/md19060349

Agarkova, I. V., Lane, L. C., Dunigan, D. D., Quispe, C. F., Duncan, G. A., Milrot, E., Minsky, A., Esmael, A., Ghosh, J. F., Van Etten, J. L. 2021 Identification of a chlorovirus PBCV-1 protein involved in degrading the host cell wall during virus infection. Viruses-Basel, 13, 782 (article ID).
Keywords: Chlorella; Phycodnaviridae; chlorovirus; PBCV-1; algal lytic activity; cell wall degrading activity; alginate-like lyase
Strain(s): 2540, 2541
PubMed: 33924931
DOI: 10.3390/v13050782

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