Recommendations for the preservation of environmental samples in diatom metabarcoding studies

Article title
Abstract
Key Words
Introduction
Materials and methods
- Site selection and sampling
- Sample preservation methods
- Storage duration and DNA extraction
- DNA quality and quantity
- PCR amplification and sequencing
- Bioinformatics
- Statistical analyses
- Diatom indices calculation
- Data availability
Results
- DNA quality and quantity
- Diatom assemblage diversity
- Diatom assemblage composition
- Ecological quality index for freshwater sites
Discussion
- Sample preservation: a robust first step in diatom metabarcoding
- Towards a standardised and user-friendly method
Conclusion and perspectives
Acknowledgements
References
Supplementary materials

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