Monitoring harmful microalgal species and their appearance in Tokyo Bay, Japan, using metabarcoding

Article title
Abstract
Key Words
Introduction
Methods
- Sampling and DNA extraction
- Paired-end library preparation, sequencing and bioinformatics
- HAB species selection
- Statistical analysis
- Association rule-based time-series analysis
Results
- Environmental parameters
- Overview of detected eukaryotes and prokaryotes
- Number of HAB species/genera detected and appearance patterns
- Statistical analysis
- Association rule-based analysis of time-series data
Discussion
- HAB species/genera detected by metabarcoding and HTS and by light microscopy
- HAB species/genera appearance patterns and correlation with environmental parameters
- HAB species/genera appearance patterns and correlation with other species/genera
Conclusions
Acknowledgements
References
Supplementary materials

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