It depends on how your 24 port hub is built internally. The more balanced hub tree is the better. E.g. best way to build 16 port hub out of five 4-port chips is to have one serving as root to connect 4 more to each of its ports, giving total port count 16, and only adding 2 levels to hub chain depth. Unfortunately, many vendors don't do it that way - e.g. Rosonway RSH-A16 USB tree is not balanced, it actually adds 4 to tree depth.

Per USB standard, hub chain depth is limited to 7. Considering that you will attach your hubs to some internal hubs, total depth can easily reach that limit, and something will break.

Because of that, I recommend looking for true 7 port or higher count hubs (I once saw true 16 port hub chip, but it didn't support PPPS). My all time favorite is Dlink DUB-H7 (old silver model, USB2 only). Best way to add more ports was buying multiple 7 port hubs and attaching them as close to root USB hubs as possible (ideally directly into root hub).

Note that when it comes to USB3 hubs, it all becomes a lot more complicated if you need high port count and make it really reliable.

I recommend reading this article if you want to really understand limitations https://acroname.com/blog/how-many-usb-devices-can-i-connect. Note that those limits are imposed by your host hardware controllers, not by USB hubs.