Alterations in the mutagenicity and mutation spectrum induced by benzo[a]pyrene instilled in the lungs of gpt delta mice of various ages

Aoki, Yasunobu; Hashimoto, Akiko H.; Sugawara, Yoshiki; Hiyoshi-Arai, Kyoko; Goto, Sataro; Masumura, Kenichi; Nohmi, Takehiko

doi:10.1186/s41021-015-0004-x

BMC is moving to Springer Nature Link. Visit this journal in its new home.

BMC

Part of Springer Nature

Genes and Environment

Table 3 DNA sequence analysis of gpt mutations obtained from the lung of B[a]P-instilled and control mice

From: Alterations in the mutagenicity and mutation spectrum induced by benzo[a]pyrene instilled in the lungs of gpt delta mice of various ages

Type of mutation	Nucleo-tide #	Sequence		Change		Amino acid change			Number
									Control			B[a]P
									Months			Months
									3^d	11	24	3^e	11	24
Base substitution
Transition
G:C → A:T	26	tGg	→	tAg	Trp	→	Stop	1	2^a	1
27	tgG	→	tgA	Trp	→	Stop	1	1
37	Cag	→	Tag	Gln	→	Stop	1	2	1
58	Gca	→	Aca	CpG	Ala	→	Thr	1
64	Cga	→	Tga	CpG	Arg	→	Stop	1	5^b	1	5^b	1
86	tGg	→	tAg	Trp	→	Stop	3^b	1
87	tgG	→	tgA	Trp	→	Stop	2^a
92	gGc	→	gAc	Gly	→	Asp	2^a
110	cGt	→	cAt	CpG	Arg	→	His	5^c	4	3^b	4^b	6^a
115	Ggt	→	Agt	CpG	Gly	→	Ser	1	5^b	2	1	2^a	1
116	gGt	→	gAt	Gly	→	Asp	2	2^a	1
128	gGt	→	gAt	Gly	→	Asp	1	2	2
176	tGt	→	tAt	Cys	→	Tyr	1
262	Gat	→	Aat	Asp	→	Asn	1
274	Gat	→	Aat	Asp	→	Asn	1	1	1
281	gGt	→	gAt	Gly	→	Asp	1
284	gGt	→	gAt	Gly	→	Asp	1
367	Gat	→	Aat	Asp	→	Asn	1
401	tGg	→	tAg	Trp	→	Stop	2^a	4^a	1
402	tgG	→	tgA	Trp	→	Stop	1	1
406	Gaa	→	Aaa	Glu	→	Lys	3^b
416	tGg	→	tAg	Trp	→	Stop	1
417	tgG	→	tgA	Trp	→	Stop	1	1	4^b
418	Gat	→	Aat	Asp	→	Asn	2^a	2^a	1	2^a	2^a
A:T → G:C	25	Tgg	→	Cgg	Trp	→	Arg	1
41	aTc	→	aCc	Ile	→	Thr	1
56	cTc	→	cCc	Leu	→	Pro	1	8^a	1
149	cTg	→	cCg	Leu	→	Pro	1	1
415	Tgg	→	Cgg	Trp	→	Arg	1
Transversion
G:C → T:A	3	atG	→	atT	Met	→	Ile	1
7	Gaa	→	Taa	CpG	Glu	→	Stop	1
15	taC	→	taA	Tyr	→	Stop	1
26	tGg	→	tTg	Trp	→	Leu	1
37	Cag	→	Aag	Gln	→	Lys	1
79	Gaa	→	Taa	Glu	→	Stop	1
92	gGc	→	gTc	Gly	→	Val	1
101	gCc	→	gAc	Ala	→	Asp	1
108	agC	→	agA	Ser	→	Arg	2
110	cGt	→	cTt	CpG	Arg	→	Leu	1
115	Ggt	→	Tgt	CpG	Gly	→	Cys	2	1	2^a
116	gGt	→	gTt	Gly	→	Val	2^a	1
140	gCg	→	gAg	CpG	Ala	→	Glu	1	1	3^a	1
143	cGt	→	cTt	CpG	Arg	→	Leu	8^a	1
145	Gaa	→	Taa	Glu	→	Stop	1	1	1
185	aGc	→	aTc	Ser	→	Ile	1	1
186	agC	→	agA	Ser	→	Arg	1
189	taC	→	taA	CpG	Tyr	→	Stop	1	3^a	1
208	Gag	→	Tag	CpG	Glu	→	Stop	3	1
244	Gaa	→	Taa	CpG	Glu	→	Stop	1	2^a	2	1	1
262	Gat	→	Tat	Asp	→	Tyr	1
281	gGt	→	gTt	Gly	→	Val	1
304	Gaa	→	Taa	Glu	→	Stop	1	1	1
320	gCg	→	gAg	CpG	Ala	→	Glu	1	1
401	tGg	→	tTg	Trp	→	Leu	2^a
402	tgG	→	tgT	Trp	→	Cys	1	1	4^b
406	Gaa	→	Taa	Glu	→	Stop	2^a	1	3^b
409	Cag	→	Aag	Gln	→	Lys	1
413	cCg	→	cAg	CpG	Pro	→	Gln	1	1
G:C → C:G	6	agC	→	agG	CpG	Ser	→	Arg	1
50	cGt	→	cCt	CpG	Arg	→	Pro	1
110	cGt	→	cCt	CpG	Arg	→	Pro	1
112	Ggc	→	Cgc	Gly	→	Arg	1	1
125	cCg	→	cGg	CpG	Pro	→	Arg	1	2^a
127	Ggt	→	Cgt	Gly	→	Arg	1
130	Gcg	→	Ccg	Ala	→	Pro	1	1
139	Gcg	→	Ccg	Ala	→	Pro	1
186	agC	→	agG	Ser	→	Arg	2	1	1
206	cGc	→	cCc	CpG	Arg	→	Pro	1
280	Ggt	→	Cgt	CpG	Gly	→	Arg	1	1
289	Gcg	→	Ccg	Ala	→	Pro	1
290	gCg	→	gGg	CpG	Ala	→	Gly	1
340	Gca	→	Cca	CpG	Ala	→	Pro	1	1	1	1	4^b	1
413	cCg	→	cGg	CpG	Pro	→	Arg	1
418	Gat	→	Cat	Asp	→	His	1
442	Cca	→	Gca	Pro	→	Ala	1	1
A:T → T:A	10	Aaa	→	Taa	Lys	→	Stop	1
25	Tgg	→	Agg	Trp	→	Arg	2^a	1
44	caT	→	caA	His	→	Gln	1
88	Aaa	→	Taa	Lys	→	Stop	2
146	gAa	→	gTa	Glu	→	Val	3^a
164	gTc	→	gAc	Val	→	Asp	2
187	Tac	→	Aac	Tyr	→	Asn	1
365	gTt	→	gAt	Val	→	Asp	1
375	taT	→	taA	Tyr	→	Stop	1
419	gAt	→	gTt	Asp	→	Val	2^a
458	tAa	→	tTa	Stop	→	Leu	1
459	taA	→	taT	Stop	→	Tyr	1
A:T → C:G	1	Atg	→	Ctg	Met	→	Leu	1
56	cTc	→	cGc	Leu	→	Arg	1	1
106	Agc	→	Cgc	Ser	→	Arg	1	1
134	tTa	→	tGa	Leu	→	Stop	1
312	taT	→	taG	Tyr	→	Stop	1
419	gAt	→	gCt	Asp	→	Ala	1	1
ᅟ
Deletion	8–12	gAAAAAt					→	gAAAAt	1	1	1
−1 base	13	aTa					→	aa	1
26–28	tGGGa					→	tGGa	1	1
34–35	gTTg					→	gTg	1
67	aCt					→	at	1
86–87	tGGa					→	tGa	1
101–102	gCCg					→	gCg	1
114	gCg					→	gg	1
124–125	aCCg					→	aCg	1
126–128	cGGGt					→	cGGt	1	1
129	gTg					→	gg	1
170–171	aCCg					→	aCg	1	2^a
176	tGt					→	tt	1
198	aCa					→	aa	1
217	aGt					→	at	1
244	cGa					→	ca	1
247–248	aGGc					→	aGc	1
249	gCt					→	gt	1
-	266	gAc					→	gc	1
270–271	tGGt					→	tGt	2^a	1
278–279	aCCg					→	aCg	1
285	gTa					→	ga	1
293–294	gTTg					→	gTg	1
315–318	cAAAAg					→	cAAAg	1
319	aGc					→	ac	1	1
332–333	aCCa					→	aCa	1
401–402	tGGa					→	tGa	1
416–418	tGGGa					→	tGGa	1	1	1
442–443	gCCa					→	gCa	1
>2	107–109	aGCCg					→	ag	2
114–120	gCGGTCTGg					→	gg	1
129–139	gTGCGTTACTGGc					→	gc	2
140–152	gCGCGTGAACTGGGt					→	gt	1
161–442	1
243–248	gCGAAGGc					→	gc	1
300–306	tTCGTGAAa					→	ta	1
375–380	aTGTTGTt					→	at	2
Insertion	25	cTg					→	cTTg	1
75	ct					→	cAt	3^a
124	ac					→	aTc	2^a
136	aCt					→	aCCt	1
223–225	gAAAc					→	gAAAAc	1
229	cGc					→	cGGc	1
286	gt					→	gATACCGGTGGt	1
335–337	aTCTt					→	aTCTTCTt	1
362	cTg					→	cTTg	1
390	cc					→	cTc	1
392–393	cAAg					→	cAAAg	1
401–402	tGGa					→	tGGGa	1	2
Other	26–27	tGGg					→	tTg	1
59–60	gCAa					→	gGa	1
100–102	tGCCg					→	tTg	1
140–141	gCGc					→	gAAc	1
304	tGa					→	tAAa	1
Total	38	92	43	42	79	49

^{a, b, and c}Mutations found in 2, 3, and 4 different mice, respectively
^dCombined data of our previous studies [11, 17, 18]
^eOur previous data [11]
CpG: mutation at CpG site

Back to article page

Genes and Environment

ISSN: 1880-7062

Contact us

Submission enquiries: Access here and click Contact Us
General enquiries: journalsubmissions@springernature.com