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AUTOMATED ANNOTATION OF NGS TRANSCRIPTOME DATA USING ISGA AND ERGATIS Chris Hemmerich Center for Genomics and Bioinformatics Indiana University, Bloomington, Indiana
Keeping up with next-generation sequencing data requires the use of automated analysis pipelines!
Ergatis is a(n awesome) workflow management tool for designing and running such pipelines! http://ergatis.sourceforge.net/! Ergatis: A web interface and scalable software system for bioinformatics workflows! Orvis et al. (2010) Bioinformatics !
ISGA is a Biologistʼs tool for running and customizing Ergatis pipelines previously designed by experts! http://isga.cgb.indiana.edu/! An Ergatis-based prokaryotic genome annotation web server! Chris Hemmerich, Aaron Buechlein, Ram Podicheti, Kashi Revanna & Qunfeng Dong, (2010) Bioinformatics!
INTEGRATIVE SERVICES FOR GENOMIC ANALYSIS Account system protects user data and enables easy retrieval of previous results Integrated visualization, query, and analysis tools to explore pipeline results File upload and download managed through browser ISGA pipelines can be run in Ergatis
TRANSCRIPTOME PIPELINE !"#$%&"'()*+,-.,/0,$&,- .,/0,$&,-60#2')7-8'2),"- 9?@.!<- 9?@.!<- 9?@.!A- <=9>-$"- 12'3*4'&5,"- IJK.!- .,2L- 9?@.!4- 4H1.>!K-.=@<- 1H84",I'&)*"- 92#%):;1- !9?@.!<- !#D*$*+7- 1")M*F=?NO9- EF,3#$G- ?'(*4B-.'3$#24-C-!#"3,)4- H,L,",$&,- ;,$*+,- 1")M*F=?-
Transcriptome pipeline is running in Ergatis. We are optimizing performance and evaluating additional functionality.! isga-users@sourceforge.net!
CREDITS Qunfeng Aaron Ram Kashi Jeong-Hyeon Dong Buechlein Podicheti Revanna Choi Haixu Tang