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. 2024 May 6;24(1):57.
doi: 10.1186/s12862-024-02244-z.

Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences

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Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences

Bahareh Nowruzi et al. BMC Ecol Evol. .

Abstract

Background: Complex descriptions of new strains of cyanobacteria appear very frequently. The main importance of these descriptions concerns potential new substances that they could synthesise, as well as their different properties as a result of their different ecological niches. The main gene used for these descriptions is 16 S with ITS or whole genome sequencing. Neowestiellopsis persica represents a unique example of the influence of ecology on morphological changes, with almost identical 16 S identity. Although our previously described Neowestiellopsis persica strain A1387 was characterized by 16 S analysis, we used different molecular markers to provide a way to separate strains of this genus that are closely related at the genetic level.

Materials and methods: In order to conduct an in-depth study, several molecular markers, namely psbA, rpoC1, nifD, nifH and cpcA were sequenced and studied in Neowestiellopsis persica strain A1387.

Results: The results of the phylogenetic analysis, based on cpcA, showed that the studied strain A 1387 falls into a separate clade than N. persica, indicating that this signature sequence could be a useful molecular marker for phylogenetic separation of similar strains isolated in the future.

Conclusions: Analysis of strain A1387 based on gene differences confirmed that it is a Neowestiellopsis strain. The morphological changes observed in the previous study could be due to different ecological and cultivation conditions compared to the type species. At the same time, the sequences obtained have increased our understanding of this species and will help in the future to better identify strains belonging to the genus Neowestiellopsis.

Identifying the cpcA gene as a useful molecular marker for species identification in the genus Neowestiellopsis.Characterizing new sequences of genes nifH, nifD, cpcA, psbA in the genus Neowestiellopsis.First in-depth study of Neowestiellopsis persica A1387 using functional genes.

Keywords: Neowestiellopsis persica, cpcA; Cyanobacteria; Phylogenetic analysis.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Morphological characterization of NeowestiellopsisPersica A1387. With increasing age there are significant increases in the number of main and branching filaments terminating in an empty sheath (a). Unilateral T-type branches arise from the main filament (b); erect true branches (with T-type branching) (c) usually unilateral (d). Sometimes bilateral branching origins from one (e) or two near cells (f). Sometimes two near cells are separated by a heterocyte (g). Moreover, the studied strain may eventually differentiate a series of spherical, thick-walled cells that are akinetes (h), 6.25 μm length ×ばつ 3.75–5 μm width. Heterocytes are intercalary (i) and could be found near the branch (j). Sometimes Hormogonia are formed at the end of a branch by one cell (k), and also directly on the main trichomes (l). Reproduction occurs via monocyte formation, which is a spherical cell, 3.5–5. 5 μm of diameter (n)
Fig. 2
Fig. 2
Phylogenetic tree constructed from nucleotide sequences of the cpcA gene: Bootstrap values are shown besides each branch, bootstrap values lower than 30 are not shown. The sequence of the studied strain is highlighted in red
Fig. 3
Fig. 3
Phylogenetic tree constructed from nucleotide sequences of the nifD gene: Bootstrap values are shown besides each branch, bootstrap values lower than 30 are not shown. The sequence of the studied strain is highlighted in red
Fig. 4
Fig. 4
Phylogenetic tree constructed from nucleotide sequences of the nifH gene: Bootstrap values are shown besides each branch, bootstrap values lower than 30 are not shown. The sequence of the studied strain is highlighted in red
Fig. 5
Fig. 5
Phylogenetic tree constructed from nucleotide sequences of the psbA gene: Bootstrap values are shown besides each branch, bootstrap values lower than 30 are not shown. The sequence of the studied strain is highlighted in red
Fig. 6
Fig. 6
Phylogenetic tree constructed from nucleotide sequences of therpoCl gene: Bootstrap values are shown besides each branch, bootstrap values lower than 30 are not shown. The sequence of the studied strain is highlighted in red.

References

    1. Hammerschmidt K, et al. The order of trait emergence in the evolution of cyanobacterial multicellularity. Genome Biol Evol. 2021;13(2):evaa249. doi: 10.1093/gbe/evaa249. - DOI - PMC - PubMed
    1. Gugger MF, Hoffmann L. Polyphyly of true branching cyanobacteria (Stigonematales) Int J Syst Evol MicroBiol. 2004;54(2):349–57. doi: 10.1099/ijs.0.02744-0. - DOI - PubMed
    1. Sciuto K, et al. Polyphasic approach and typification of selected Phormidium strains (Cyanobacteria) Cladistics. 2012;28(4):357–74. doi: 10.1111/j.1096-0031.2011.00386.x. - DOI - PubMed
    1. Seo P-S, Yokota A. The phylogenetic relationships of cyanobacteria inferred from 16S rRNA, gyrB, rpoC1 and rpoD1 gene sequences. J Gen Appl Microbiol. 2003;49(3):191–203. doi: 10.2323/jgam.49.191. - DOI - PubMed
    1. Boyer SL, Flechtner VR, Johansen JR. Is the 16S–23S rRNA internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in cyanobacteria Molecular biology and evolution, 2001. 18(6): pp. 1057–1069. - PubMed

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