Tools for Advanced DNA and Protein Technologies
TopoTaqTopoTaq DNA Polymerase. The most robust enzyme for PCR amplification
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Whole
Genome Direct (WGD) Sequencing Technology
Methanopyrus kandleri
The Complete Genome
of the Hyperthermophile
Methanopyrus kandleri AV19
and Monophyly of
Archaeal Methanogens Slesarev,A.I., Mezhevaya,K.V., Makarova,K.S.,
Polushin,N.N., Shcherbinina,O.V.,
Shakhova,V.V., Belova,G.I., Aravind,L.,
Natale,D.A., Rogozin,I.B., Tatusov,R.L.,
Wolf,Y.I.,
Stetter,K.O., Malykh,A.G.,
Koonin,E.V. and Kozyavkin,S.A. (2002)
Proc. Natl. Acad. Sci. U.S.A. 99, 4644-4649
EVOLUTION: Stripped Down to the Bare Essentials
Science 296, 431 (2002) Editors' Choice A
HAPPY MARRIAGE: advancing DNA polymerases with DNA topoisomerase
Trends in Biotech 20, 491 (2002) Journal Club
Major breakthrough in DNA sequencing technology
Direct Automated Sequencing of Bacterial Genomic DNA
At the Ninth International Genome Sequencing and Analysis Conference
Dr. Bruce Roe from the University of Oklahoma presented his group's data on the successful automated sequencing of
non-clonable and
non-PCRable regions of Streptococcus pyogenes (1.92 Mb). The scientists used ThermoFidelase I and a new sequencing kit to sequence genomic DNA directly. This took less than a week from receiving new reagents to presenting the most stunning results to 1200 attendees of the conference. "It completely changed our finishing strategy" - said Dr. Roe.
Successful Sequencing of Non-sequenceable DNAAt the 10th Annual Cold Spring Harbor Meeting on Genome Mapping and Sequencing Dr. Elaine Mardis of Washington University presented data on using ThermoFidelase I in large scale sequencing projects. "This approach has proven successful in sequencing through stop regions of M13 or pUC subclones, and PCR products" - concluded Dr. Mardis.
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