Questions about clean up of PCR product
Midgard's Vurm
the-wyrm at excite.com
Wed Mar 31 16:59:41 EST 1999
In article <7dt7ek$rr02 at hkusud.hku.hk>, "Wilson" <netson at hotmail.com> wrote:
: Dear all,
: I would like to know the effective and economic way(s) to clean up PCR
: product. I always use the following procedures: precipitate PCR product,
: then have a agarose gel electrophoresis, cut out the target DNA band and
: retrieve DNA by GeneClean. Is there any other way (such as special
: precipitation methods) to remove impurities such as primers and dNTPs
: effectively?
You can save yourself some dough by using DE81 paper instead of GeneClean.
And skip precipitation of the product if you are doing gel e/phoresis
afterwards.
Cheers,
V.
--
V. Svetlov, Ph. D.
McArdle Laboratory for Cancer Research
University of WI, Madison
1400 University Ave.
Madison, WI 53706
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