protein expression in PLysS
Anant Patkar
apatkar at biosci.cbs.umn.edu
Sat Mar 27 20:14:17 EST 1999
We have also observed leaky expression in E. coli BL21(DE3) with
pET23-based
vector. There was also significant plasmid stability problem. Because
of the leaky expression, only 60% of the cells would have plasmid after
reaching stationary phase. Has anyone seen this?
Anant Patkar
University of Minnesota
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