In article <36F2ACD1.FF0120BC at leonardo.ls.huji.ac.il>, yoramg2 at LEONARDO.LS.HUJI.AC.IL (Yoram Gerchman) wrote: > Greetings netters. > Dose anyone have a good method for digest 5' overhang ? I tried Mung > Bean but it cut into my gene. Will it be better in lower temperature > (11C) a-la T4 polymerize? With mung bean, make a titration series (several 1/5s) of the enzyme and incubate at RT. This worked for me. Good luck, jorg