RNA injection into the mouse oocytes
Sandra Wong
syywong at hkusua.hku.hk
Wed Mar 17 03:26:27 EST 1999
Dear user,
I am going to do the microinjection of RNA into the mouse oocytes. Does
anyone has a protocol that I can follow?
My plasmid is T7-IRES-Cre. I need a protocol for RNA synthesis for
injection. If you have the protocol. Kindly please send this to me.
I also have few questions to ask. The RNA that I synthesis is dissolve in
what kind of solution for injection. Do I need to cap the RNA for the
stability?
Best regards,
Sandra Wong
email address: syywong at hkusua.hku.hk
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