In article <7ck8d8$s22$1 at nnrp1.dejanews.com>, ladasky at my-dejanews.com writes >>The long PCR protocols typically use between 1% and 10% Taq polymerase in >conjunction with Vent (NEB) or Pwo (Boehringer) or Pfu (Stratagene). This >way you get the error correction of the high-fidelity enzym and the proces- >sivity of Taq. Wrong way around. 1-10% proof-reading to 90-99% Taq. Duncan -- The problem with being on the cutting edge is that you occasionally get sliced from time to time.... Duncan Clark DNAmp Ltd. Tel: +44(0)1252376288 FAX: +44(0)8701640382 http://www.dnamp.com http://www.genesys.demon.co.uk