Purifying Antibodies using affinity support
Wolfgang Schechinger
Wolfgang.Schechinger at med.uni-tuebingen.de
Tue Mar 9 13:16:39 EST 1999
Dima,
Suppose the periodate protocol is oxidizing cis diols into
bis aldehydes and reduce the imines formed after coupling with
cyanoborohydride to amines. And clening leftover CHOs with boranate.
But this procedure above probably might kill any active enzyme. Is
there a way to prevent this?
You remember the exact protocol by chance?
Wolfgang
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Wolfgang Schechinger
Pathobiochemistry Dept.
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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