precipitating proteins w/ ethanol
Jee-Yeong Jeong
jeong at biochem.purdue.edu
Fri Mar 5 10:52:57 EST 1999
Hi. The EtOH ppt is of course a good choice, but it is sometimes
inconvenient; for example, if you have 0.8ml of sample in 1.5ml tube, you
need to transfer the sample to a larger tube to add 2-3 Vol of EtOH. In this
case I usually use the TCA precipitation followed by acetone washing. This
might be a classical method for protein ppt before SDS-PAGE, but it worked
well always. If you need detailed information let me know.
Katherine Walstrom wrote:
> I have some dilute protein samples that I need to concentrate and run on
> an SDS-PAGE gel. I can't find a detailed protocol for precipitating
> proteins, although what I've read suggests that ethanol works well. I
> want to precipitate all the proteins, not just selectively precipitate
> some of them. Does anyone have a general protocol for this?
>> Thanks much.
>> Katherine M. Walstrom, PhD
> Division of Natural Sciences
> New College of USF
> 5700 N. Tamiami Trail
> Sarasota, FL 34243
> kwalstro at sar.usf.edu
--
===================================================
Jee-Yeong Jeong
Graduate Research Assistant
Department of Biochemistry
Purdue University
Hansen Life Science Research Building, Room 225
West Lafayette, IN 47907
Tel (765) 494-6591 Fax (765) 494-7897
Email: jeong at biochem.purdue.edu
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