Darren, you don't write in what host you express your protein. If it's E. coli, you could try 2 to 6M urea or guanidinium salts for solubilizing your inclusion bodies. Wolfgang ----- usual disclaimers apply * This message is RNAse free - please don't touch! ----- Wolfgang Schechinger Pathobiochemistry Dept. University of Tuebingen, Germany email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm ------- *unsolicited mail in *NOT* appreciated