Amino acid analysis problems
ummanley at cc.UManitoba.CA
ummanley at cc.UManitoba.CA
Thu Mar 4 11:28:14 EST 1999
Sorry.....
I express in the E.coli strain BL21(DE3)pLysS. Actually I use a 4M urea/
SDS mixture for solubilization (separately the urea and SDS are
inefficient at solubilizing my protein, the best detergent is
dodecylmaltoside.....but its very expensive). The protein is a natural
E.coli membrane protein, and there is no evidence of inclusion bodies
(even EM doesn't show anything).
Do you suggest removing the SDS instead of the urea for AAA?
Thanks,
Darren
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
{ }
{ Darren Manley Department of Chemistry }
{ phone (204) 474-6697 University of Manitoba }
{ fax (204) 474-7608 Winnipeg, MB., R3T-2N2 }
{ email ummanley at cc.umanitoba.ca Canada }
{ }
{ }
{ Quantum Mechanics: The dreams stuff is made of. }
{ }
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
More information about the Methods
mailing list
Send comments to us at biosci-help [At] net.bio.net