Platform: Win32 Availability: Free for non-commercial users only. PRESTA (PRomoter EST Association) is a software tool designed for efficient recovery of characterised and partially verified promoters from GenBank and EMBL libraries. PRESTA algorithm examines the putative GenBank/ EMBL promoters and automatically removes most of the poorly annotated entries. The remaining records are connected to ESTs (Expressed Sequence Tags) through the use of a high-stringency Blast search. The frequency and source of recovered ESTs provide an estimate of the activity and expression pattern of the promoter, whereas the ESTs 5' ends assist in the transcription start site verification. "http://baloun.entu.cas.cz/presta/" PRESTA web site and the on-line database of PRESTA-extracted promoters. "http://www.genomebiology.com/2002/3/9/research/0050" published description of PRESTA