GINMU

GINMU http://ginmu.naramed-u.ac.jp:80/dspace DSpaceデジタルリポジトリシステムは、デジタル研究資料の捕捉・保管・索引化・保存・配布を行います。 2025年10月14日 09:04:25 GMT 2025年10月14日T09:04:25Z Immunosuppressant-Induced Alteration of Gut Microbiota Causes Loss of Skeletal Muscle Mass : Evidence from Animal Experiments Using Mice and Observational Study on Humans http://hdl.handle.net/10564/4466 タイトル: Immunosuppressant-Induced Alteration of Gut Microbiota Causes Loss of Skeletal Muscle Mass : Evidence from Animal Experiments Using Mice and Observational Study on Humans 著者: Tomizawa, Mitsuru; Hori, Shunta; Yoneda, Tatsuo; Maesaka, Fumisato; Onishi, Sayuri; Shimizu, Takuto; Onishi, Kenta; Morizawa, Yosuke; Gotoh, Daisuke; Nakai, Yasushi; Miyake, Makito; Torimoto, Kazumasa; Tanaka, Nobumichi; Fujimoto, Kiyohide 抄録: Background/Objectives: The number of older adults requiring a kidney transplant (KT) is increasing; hence, postoperative sarcopenia prevention is necessary. KT recipients require permanent oral immunosuppressants (ISs), and the gut microbiota (GM) plays a role in various systemic diseases. However, few studies have evaluated post-kidney transplantation frailty and the associations among ISs, GM, and muscle mass alterations. Therefore, we investigated the effects of ISs on GM and skeletal muscle mass in mice and human KT recipients. Methods: Mice were treated with six different ISs, and their skeletal muscle mass, GM diversity, and colonic mucosal function were assessed. Human KT recipients and donors were monitored before and after surgery for 1 year, and GM diversity was evaluated before and 1 month after surgery. Results: The abundance of Akkermansia, crypt depth, and mucin 2 expression were lower in tacrolimus- and prednisolone-treated mice. The psoas muscle volume changes at 1 month and 1 year after surgery were lower in KT recipients than in donors. Furthermore, the beta diversity was significantly different between the operative groups (p = 0.001), and the KT group showed the lowest Shannon index. Conclusions: The findings of this study indicate potential links among ISs, GM, and muscle mass decline. Further investigation is required to improve therapeutic strategies and patient outcomes. 内容記述: 権利情報:© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/). 2025年1月31日 15:00:00 GMT http://hdl.handle.net/10564/4466 2025年01月31日T15:00:00Z Post-Illumination Pupil Response and Sleep Quality in Patients With Glaucoma : The LIGHT Study http://hdl.handle.net/10564/4465 タイトル: Post-Illumination Pupil Response and Sleep Quality in Patients With Glaucoma : The LIGHT Study 著者: Jimura, Hironobu; Yoshikawa, Tadanobu; Obayashi, Kenji; Miyata, Kimie; Saeki, Keigo; Ogata, Nahoko 抄録: PURPOSE. This study aimed to investigate whether intrinsically photosensitive retinal ganglion cell function evaluated using post-illumination pupil response (PIPR) in patients with glaucoma is associated with sleep quality. METHODS. This cross-sectional study measured the PIPR in 138 patients with glaucoma (mean age, 70.3 years) using pupil diameter after red and blue light exposure. The net PIPR change was classified into three groups according to tertiles (i.e., low, intermediate, and high groups), with lower net PIPR change indicating lower intrinsically photosensitive retinal ganglion cell (ipRGC) function. Subjective and objective sleep qualities were assessed using the Pittsburgh Sleep Quality Index (PSQI) questionnaire and actigraphy, respectively, with a total PSQI score of ≥6 indicating sleep disturbance. RESULTS. The prevalence of subjective sleep disturbance significantly increased with decreasing tertile groups of net PIPR change (P = 0.036). Subgroup analysis obtained the same results in the severe glaucoma group (P = 0.004) but not in the non-severe glaucoma group. In the severe glaucoma group, multivariable logistic regression analysis adjusted for potential confounders showed a higher odds ratio for subjective sleep disturbance in the low-tertile group of net PIPR compared with the high-tertile group (odds ratio = 6.22; 95% confidence interval, 1.76–21.90; P = 0.004). Significant associations between PIPR and objective sleep quality (total sleep time, sleep efficiency, and wake after sleep onset) were found in the severe glaucoma group (P = 0.015, P = 0.013, and P = 0.015, respectively). CONCLUSIONS. The PIPR in patients with glaucoma was significantly associated with decreased sleep quality, independent of potential confounders. 内容記述: 権利情報:© 2023 The Authors. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. 2023年8月31日 15:00:00 GMT http://hdl.handle.net/10564/4465 2023年08月31日T15:00:00Z Anti‐mitochondrial M2 antibody‐positive myositis may be an independent subtype of autoimmune myositis http://hdl.handle.net/10564/4464 タイトル: Anti‐mitochondrial M2 antibody‐positive myositis may be an independent subtype of autoimmune myositis 著者: Nishimori, Yukako; Tanboon, Jantima; Oyama, Munenori; Motegi, Haruhiko; Tomo, Yui; Oba, Mari; Yamanaka, Ai; Sugie, Kazuma; Suzuki, Shigeaki; Hayashi, Shinichiro; Noguchi, Satoru; Nishino, Ichizo 抄録: It is still unknown whether anti-mitochondrial M2 antibody (AM2A)-positive myositis is an independent subtype of autoimmune myositis (AIM). As such, the aim of this study is to better characterize the clinicopathological features in a large cohort of patients. This study utilized the muscle biopsy samples from AM2A-positive patients, which were sent to the National Center of Neurology and Psychiatry for diagnostic purposes from January 2008 to December 2020. The clinicopathologic information of 201 patients were compared with those who were diagnosed with immune-mediated necrotizing myopathy (IMNM), anti-synthetase syndrome, or dermatomyositis. AM2A-positive patients had the longest pre-biopsy disease duration (PBDD) at 48.7 ± 63.0 months and the highest frequency of arrhythmia of 51.1%. Necrotic and/or regenerating fibers were seen in 93.5% and membrane attack complex sarcolemmal deposits were noted in 43.3%, similar to IMNM. Furthermore, AM2A-positive patients with shorter PBDD showed more CD8-positive lymphocyte infiltrates. Clinically, shorter PBDD was associated with higher serum creatine kinase levels, whereas longer PBDD was associated with a higher frequency of arrhythmia. Principal component analysis separated disease groups with high weight of muscle pathology components on two-dimensional plotting, although AM2A-positive myositis and IMNM partly overlapped. On logistic regression model analysis, we obtained high sensitivity (0.846) and specificity (0.842) for distinguishing them using clinical and pathological variables. This largest cohort study suggests that AM2A-positive myositis may be an independent subtype of AIM characterized by a chronic myositis with IMNM-like pathology, along with a high prevalence of cardiac involvement and respiratory muscle weakness. 内容記述: 権利情報:© 2025, Springer-Verlag GmbH Germany, part of Springer Nature 2025年1月31日 15:00:00 GMT http://hdl.handle.net/10564/4464 2025年01月31日T15:00:00Z Intra-arterial Therapy Using Micellar Nanoparticles Incorporating SN-38 in a Rat Pancreatic Tumor Model http://hdl.handle.net/10564/4462 タイトル: Intra-arterial Therapy Using Micellar Nanoparticles Incorporating SN-38 in a Rat Pancreatic Tumor Model 著者: Maeda, Shinsaku; Sato, Takeshi; Nishiofuku, Hideyuki; Toyoda, Shohei; Taiji, Ryosuke; Matsumoto, Takeshi; Chanoki, Yuto; Tachiiri, Tetsuya; Kunichika, Hideki; Sho, Masayuki; Tanaka, Toshihiro 抄録: Purpose To evaluate advantages of micellar nanoparticles encapsulating SN-38, a biologically active metabolite of irinotecan, in intraarterial therapy for pancreatic cancer. Materials and Methods Rat pancreatic cancer cells (DSL-6A/C1) were implanted in Lewis rats under laparotomy. This study consists of two parts. Firstly, after confirming tumor formation by ultrasonography, celiac arteriography was performed, and tumor blood supply was visually evaluated by dye injection and CT during arteriography. Secondly, 18 rats were divided into two groups; the Micellar Nanoparticles group and the Irinotecan Infusion group. Micellar nanoparticles or irinotecan was injected via the celiac artery, and SN-38 and irinotecan concentrations in the tumor, duodenum and pancreatic parenchyma, were measured at 5 min, 6 h and 24 h. Results The maximum concentration (Cmax) of SN-38 were shown at 6 h in the Micellar Nanoparticles group, while Cmax of irinotecan was shown at 5 min in the Irinotecan Infusion group. Tumor concentration in the Micellar Nanoparticles group maintained elevated for 24 h without significant decrease (P = 0.068). Conversely, a significant decrease was observed in the regular pancreas parenchyma (P = 0.006) and duodenum (P = 0.028). In the Irinotecan Infusion group, tumor irinotecan concentration significantly decreased at 24 h (P = 0.016). Conclusion Micellar nanoparticles may improve arterial infusion chemotherapy for pancreatic cancer. These nanoparticles have the potential to reduce SN-38 accumulation in duodenum, while increasing it in the tumor. Further research is warranted to validate and expand upon these findings. 内容記述: 権利情報:© Springer Science+Business Media, LLC, part of Springer Nature and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE) 2025 2025年2月28日 15:00:00 GMT http://hdl.handle.net/10564/4462 2025年02月28日T15:00:00Z