ELM - instance TRG_NLS_MonoExtN_4 in sequence P03070 at position 127

ELM

The Eukaryotic Linear Motif resource for

Functional Sites in Proteins

o Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002397	P03070 Large T antig LT_SV40	127	132	SQHSTPPKKKRKVEDPKDFP	TP	---	Simian virus 40	708

o Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0403	colocalization	in vitro	Kalderon,1984	support	certain
experimental	MI:0074	mutation analysis	in vivo/in vitro	Kalderon,1984	support	certain	FeatureDetection

o Interactions

Uniprot Id	Domain family	Domain Start	Domain End	Affinity Min/Max (µMol)	Notes
(P52293) IMA2_MOUSE	PF00514 (Arm) Armadillo/beta-catenin-like repeat	[mitab] [xml]

o Switches

This ELM instance is part of the following 2 switching mechanisms annotated at the switches.ELM resource:

SWTI000315:
ELM Switch SWTI000315
Inhibition of nuclear import of Large T antigen by phosphorylation-dependent (T124) binding of BRCA1-associated protein (BRAP).
SWTI000329:
ELM Switch SWTI000329
An intramolecular interaction between the importin beta-binding (IBB) domain and the NLS-binding pocket of Importin subunit alpha (SRP1) prevents binding of NLS cargo (e.g. Large T antigen) in the absence of Importin subunit beta-1 (KAP95) by hiding of the NLS-binding pocket. Binding of the IBB of Importin subunit alpha (SRP1) to Importin subunit beta-1 (KAP95) relieves this auto-inhibitory interaction and increases the affinity of Importin subunit alpha (SRP1) for NLS cargo.

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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