ELM - instance MOD_GSK3_1 in sequence P84022 at position 201

o Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI002176	P84022 SMAD3 SMAD3_HUMAN	201	208	QMNHSMDAGSPNLSPNPMSP	TP	---	Homo sapiens (Human)	425

o Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0788	knock out	in vivo	Wang,2009	support	certain
experimental	MI:0074	mutation analysis	in vivo/in vitro	Wang,2009	support	certain	FeatureDetection
experimental	MI:0424	protein kinase assay	in vitro	Wang,2009	support	certain	InteractionDetection

o Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000279:
ELM Switch SWTI000279
CDK8/9 phosphorylates Mothers against decapentaplegic homolog 3 (SMAD3) at T179 and S208. Phosphorylation of T179 creates a binding site for the WW domain of Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1), while phosphorylation of S208 primes SMAD3 for phosphorylation of S204 by Glycogen synthase kinase-3 beta (GSK3B). The pS204-pS208 forms a binding site for the third WW domain of E3 ubiquitin-protein ligase NEDD4-like (NEDD4L), whose second WW domain will displace the WW domain of PIN1 at the pT179-PY box site of SMAD3. This regulation couples SMAD3 activation to SMAD3 destruction in an ordered fashion. See also switch details and switch details.

o Pathways

KEGG: The sequence P84022 is implicated in the following 22 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: ELM-the Eukaryotic Linear Motif resource-2024 update. (PMID:37962385)

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