Fottea

Fottea 2021, 21(1):100-109 | DOI: 10.5507/fot.2021.003

Annamia dubia sp. nov. with a description of a new family, Geminocystaceae fam. nov. (Cyanobacteria)

Akihiro Tuji^{1, *}, Haruyo Yamaguchi², Takafumi Kataoka³, Mayumi Sato², Tomoharu Sano⁴, Yuko Niiyama¹

¹ Department of Botany, National Museum of Nature and Sciences, Amakubo 4-1-1, Tsukuba, Ibaraki 305-0005, Japan *Corresponding author e-mail: tuji@kahaku.go.jp

² Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Ibaraki 305-8506, Japan

³ Faculty of Marine Science and Technology, Fukui Prefectural University, 1-1 Gakuen-cho Obama, Fukui 917-0003, Japan

⁴ Center for Environmental Measurement and Analysis, National Institute for Environmental Studies, Ibaraki 305-8506, Japan

Our environmental DNA analysis identified Annamia-like operational taxonomic units (OTUs) from Lake Kasumigaura, Japan every summer season during the period from June 2012-May 2015. A strain from Lake Kasumigaura that agreed with an Annamia-like OTU (NIES-4383) was established with a cell-sorting machine. Based on the differences in morphology and 16S rRNA sequences, a new taxon is described: Annamia dubia Tuji, Yamaguchi et Niiyama sp. nov. A. dubia produces a microcystins that is also produced by A. toxica. Our 16S rRNA phylogenetical analysis reveals that the genus Annamia makes a monophyletic clade with the genera Geminocystis, Geminobacterium, and 'Cyanobacterium.' Although this clade agrees with Cyanobacteriaceae Komárek et al. (2014), this family is invalid based on the invalid genus Cyanobacterium and the type species Cyanobacterium stanieri. We propose the alternative name Geminocystaceae Tuji, Yamaguchi et Niiyama fam. nov. based on the genus Geminocystis.

Keywords: Cyanobacteriaceae, Chroococcales, Lake Kasumigaura, Microcystin, NGS, Phylogeny, Phytoplankton

Received: August 4, 2020; Accepted: November 29, 2020; Prepublished online: March 2, 2021; Published: April 1, 2021

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