ORCID
https://orcid.org/0009-0002-4584-3047
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan. Co-first author.
ORCID
https://orcid.org/0000-0001-9895-0028
Division of RNA and Gene regulation, Institute of Medical Science, The University of Tokyo, Tokyo, Japan. Co-first author.
ORCID
https://orcid.org/0000-0002-9203-6697
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
ORCID
https://orcid.org/0000-0002-7346-360X
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
ORCID
https://orcid.org/0000-0002-2695-588X
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan. Division of RNA and Gene regulation, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
ORCID
https://orcid.org/0000-0002-2339-2938
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
2025 Volume 101 Issue 1 Pages 41-53
Crossref Funder ID: http://dx.doi.org/10.13039/501100002241
Grant/Award number:・JPMJPR21EE
・JPMJPR20EE
Crossref Funder ID: http://dx.doi.org/10.13039/100004412
Grant/Award number:・CDA00049/2018-C
Crossref Funder ID: http://dx.doi.org/10.13039/501100001691
Grant/Award number:・JP22H02653
Crossref Funder ID: http://dx.doi.org/10.13039/100007802
Grant/Award number:・L-2020-2-008
Cell proliferation is a fundamental characteristic of organisms, driven by the holistic functions of multiple proteins encoded in the genome. However, the individual contributions of thousands of genes and the millions of protein molecules they express to cell proliferation are still not fully understood, even in simple eukaryotes. Here, we present a genome-wide translation map of cells during proliferation in the unicellular alga Cyanidioschyzon merolae, based on the sequencing of ribosome-protected messenger RNA fragments. Ribosome profiling has revealed both qualitative and quantitative changes in protein translation for each gene during cell division, driven by the large-scale reallocation of ribosomes. Comparisons of ribosome footprints from non-dividing and dividing cells allowed the identification of proteins involved in cell proliferation. Given that in vivo experiments on two selected candidate proteins identified a division-phase-specific mitochondrial nucleoid protein and a mitochondrial division protein, further analysis of the candidate proteins may offer key insights into the comprehensive mechanism that facilitate cell and organelle proliferation.