Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Regular Papers
Linking Temporal Changes in Bacterial Community Structures with the Detection and Phylogenetic Analysis of Neutral Metalloprotease Genes in the Sediments of a Hypereutrophic Lake
Shun Tsuboi, Shigeki Yamamura, Akio Imai, Takayuki Satou, Kazuhiro Iwasaki
Author information
  • Shun Tsuboi

    National Institute for Environmental Studies (NIES)

  • Shigeki Yamamura

    National Institute for Environmental Studies (NIES)

  • Akio Imai

    National Institute for Environmental Studies (NIES)

  • Takayuki Satou

    National Institute for Environmental Studies (NIES)

  • Kazuhiro Iwasaki

    National Institute for Environmental Studies (NIES)

Corresponding author

ORCID
Keywords: bacterial community structure, sediment cores, extracellular protease genes, ammonium increase, hypereutrophic freshwater lake
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2014 Volume 29 Issue 3 Pages 314-321

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  • Published: 2014 Received: May 02, 2014 Released on J-STAGE: September 17, 2014 Accepted: July 15, 2014 Advance online publication: August 13, 2014 Revised: -
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Abstract
We investigated spatial and temporal variations in bacterial community structures as well as the presence of three functional proteolytic enzyme genes in the sediments of a hypereutrophic freshwater lake in order to acquire an insight into dynamic links between bacterial community structures and proteolytic functions. Bacterial communities determined from 16S rRNA gene clone libraries markedly changed bimonthly, rather than vertically in the sediment cores. The phylum Firmicutes dominated in the 4–6 cm deep sediment layer sample after August in 2007, and this correlated with increases in interstitial ammonium concentrations (p < 0.01). The Firmicutes clones were mostly composed of the genus Bacillus. npr genes encoding neutral metalloprotease, an extracellular protease gene, were detected after the phylum Firmicutes became dominant. The deduced Npr protein sequences from the retrieved npr genes also showed that most of the Npr sequences used in this study were closely related to those of the genus Bacillus, with similarities ranging from 61% to 100%. Synchronous temporal occurrences of the 16S rRNA gene and Npr sequences, both from the genus Bacillus, were positively associated with increases in interstitial ammonium concentrations, which may imply that proteolysis by Npr from the genus Bacillus may contribute to the marked increases observed in ammonium concentrations in the sediments. Our results suggest that sedimentary bacteria may play an important role in the biogeochemical nitrogen cycle of freshwater lakes.
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© 2014 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology

This article is licensed under a Creative Commons [Attribution 4.0 International] license.
https://creativecommons.org/licenses/by/4.0/
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