Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Short Communication
Detection of Defluvicoccus-related Glycogen-accumulating Organisms in Enhanced Biological Phosphorus Removal Processes
Takashi Kondo, Yoshitaka Ebie, Satoshi Tsuneda, Yuhei Inamori
Author information
  • Takashi Kondo

    Department of Chemical Engineering, Waseda University

  • Yoshitaka Ebie

    Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies

  • Satoshi Tsuneda

    Department of Chemical Engineering, Waseda University

  • Yuhei Inamori

    Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies

Corresponding author

ORCID
Keywords: Alphaproteobacteria, Enhanced biological phosphorus removal (EBPR), Fluorescence in situ hybridization (FISH), G-bacteria, Genus Defluvicoccus
JOURNAL FREE ACCESS

2007 Volume 22 Issue 2 Pages 190-195

Details
  • Published: 2007 Received: December 16, 2006 Available on J-STAGE: June 15, 2007 Accepted: March 01, 2007 Advance online publication: - Revised: -
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Abstract
To distinguish subgroups of Defluvicoccus-related glycogen-accumulating organisms (Defluvicoccus-related G-bacteria), new oligonucleotide probes were designed. Two of these probes, DF636 and DF827, were used successfully to detect each subgroup of Defluvicoccus-related G-bacteria. The morphological differences in clusters and cells between the DF636- and DF827-binding cells were confirmed. Interestingly, DF636-binding cells showed autofluorescence under UV light. Additionally, autofluorescence was observed in some alphaproteobacterial G-bacteria, which did not bind to any of the probes designed in this study. The results suggested that the Defluvicoccus-related G-bacteria consist of several physiologically different subspecies.
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