This is usually safe to clean up manually. Windows can show duplicate startup entries when one was left behind by an installer/repair path, or when the same app is registered in two startup locations.

I would first identify where both entries come from:

Get-CimInstance Win32_StartupCommand |
 Where-Object { $_.Name -like '*UniGetUI*' -or $_.Command -like '*UniGetUI*' } |
 Select-Object Name, Command, Location

Also check the two common startup folders:

explorer shell:startup
explorer shell:common startup

If one entry is just a shortcut there, delete only the duplicate shortcut. If the duplicate is in the registry, check these locations:

reg query HKCU\Software\Microsoft\Windows\CurrentVersion\Run /s | findstr /i UniGetUI
reg query HKLM\Software\Microsoft\Windows\CurrentVersion\Run /s | findstr /i UniGetUI

The least risky cleanup path is:

1. Disable one UniGetUI entry in Task Manager > Startup apps.
2. Reboot and confirm UniGetUI still starts once.
3. If everything is fine, remove the disabled duplicate from the startup folder or registry location shown by Win32_StartupCommand.
4. In UniGetUI settings, toggle "start with Windows" off and back on if you want the app to recreate a single clean entry.

If both entries point to exactly C:\Program Files\UniGetUI\UniGetUI.exe, keeping just one is enough. I would not reinstall again unless the duplicate comes back after the settings toggle.

If this solves it, please mark this comment as the answer so other people can find it faster.

Yes, with those checks I would treat it as a Windows Settings/Startup UI glitch rather than a second real startup registration.

If Win32_StartupCommand, the startup folder, the registry, and Autoruns all show only one UniGetUI entry, there is no second startup item left to remove. Deleting and restoring the shortcut probably forced Windows Settings to refresh its cached startup-app list.

I would leave it as-is unless the duplicate comes back after a reboot or after the next UniGetUI update/repair. If it does come back, the useful report would be whether Autoruns shows one or two entries at that exact moment.