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Allow user-defined rules #7
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I tested the tool on a couple of protein sequences and for one of them the predicted DNA sequence is too complex for synthesis for both IDT and twist.
Twist has the following rules to determine whether a sequence is too complex:
- Avoid repeats of ≥ 20bp or Tm ≥ 60C
- Global GC content must be between 25% and 65%
- Avoid extreme differences in GC content within a gene (i.e. the difference in GC content between the highest and lowest 50bp stretch should be no greater than 52%)
- Minimize homopolymers
- Minimize the number/length of small repeats scattered throughout the sequence
- For HIS tags use a combination of CAC and CAT codons i.e. CACCAT...
Output sequences could be screened for such issues and regenerated if needed.