Talk:Flow-FISH

This article has 2 problems (a) it reads like an advertisement or perhaps grant proposal for a specific technique (with sections highlighting the "innovation" and "advantages"), and (b) it focuses on one specific technique (measuring telomere length) that has been called Flow-FISH while ignoring other techniques. In microbial ecology the combination of FISH and flow cytometry for enumerating bacteria based (usually) on rRNA-targeted probes is generically called Flow-FISH. However cell biologists and microbiologists are frequently ignorant of each others' fields so the authors may not be aware that microbial ecologists were combining FISH and flow cytometry as early as 1993 (DOI: 10.1002/cyto.990140205}. They may not have started using the name Flow-FISH until later on, but by 2006 it was in use for microbial applications (doi: 10.1128/AEM.02283-05). The name Flow-FISH is also applied to various other techniques for FISH targeting RNA in eukaryotic cells, using flow cytometry as a readout (see doi:10.1016/j.ab.2009年04月02日6 and references therein). — Preceding unsigned comment added by RobertM75 (talk • contribs) 20:02, 29 June 2015 (UTC) Reply

I completely agree with the above notes and will work on overhauling this page to more accurately reflect the diverse fields which Flow-FISH covers, including mRNA detection in cells as well as cytogenetics. Therazzz (talk) 15:50, 11 February 2020 (UTC) Reply

Please add the following information to ‘2. Advantages’: Telomere testing is available to support physicians with clinical diagnosis of telomere biology disorders such as dyskeratosis congenita

Please add the following information to ‘4. External links’: RepeatDx website

Explanation of issue: Additional bullet point with information currently not mentioned, that could be of public interest

References supporting change: Alter BP, Baerlocher GM, Savage SA, et al. Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita. Blood. 2007;110(5):1439-1447. doi:10.1182/blood-2007年02月07日5598. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1975834/

JLRD309 (talk) 19:36, 18 August 2020 (UTC) Reply

Sorry, but I deleted the Advantages section because it was vauge, uncited, and didn't add any new information. As for the external link, I also have to say no as we aren't supposed to add external links that primarily sell products (except for official links). Thanks for using the COI edit process! Darth Flappy ^«Talk» 12:06, 19 August 2020 (UTC) Reply