Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

Microbial Cells and Enzymes: A Century of Progress Jose L. Adrio and Arnold L. Demain Enzyme Biosensors Steven J. Setford and Jeffrey D. Newman Directed Evolution of Enzymes: How to Improve Nucleic Acid Polymerases for Biocatalysis Susanne Brakmann and Sascha Nico Stumpp L-Glutaminase as a Therapeutic Enzyme of Microbial Origin Abdulhameed Sabu, K. Madhavan Nampoothiri, and Ashok Pandey Enzymatic Production of D-Amino Acids Hemraj S. Nandanwar, Gurinder S. Hoondal, and Rakesh M. Vohra Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides Oskar Banuelos, Maria del Carmen Ronchel, Jose Luis Adrio, and Javier Velasco Food-Grade Corynebacteria for Enzyme Production Jose A. Gil, Angelina Ramos, Sirin A. I. Adham, Noelia Valbuena, Michal Letek, and Luis M. Mateos Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase Yasuhisa Asano Enzymes in Modern Detergents Susumu Ito, Tohru Kobayashi, Yuji Hatada, and Koki Horikoshi Microbial Proteases Chandran Sandhya, K. Madhavan Nampoothiri, and Ashok Pandey Proteases Produced by Halophilic Bacteria and Archaea Encarnacion Mellado, Cristina Sanchez-Porro, and Antonio Ventosa Microbial Pectinases Rupinder Tewari, Ram P. Tewari, and Gurinder S. Hoondal Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes Xin Gen Lei and Taewan Kim Glucose Dehydrogenase for the Regeneration of NADPH and NADH Andrea Weckbecker and Werner Hummel Acetate Kinase from Methanosarcina thermophila, a Key Enzyme for Methanogenesis Prabha Iyer and James G. Ferry Immobilization of Enzymes by Covalent Attachment Scott J. Novick and J. David Rozzell Preparation of an IndustrialBiocatalyst of Penicillin G Acylase on Sepabeads: Improving the Design of Penicillin G Hydrolysis Cesar Mateo, Valeria Grazu, Olga Abian, Manuel Fuentes, Gloria Fernandez-Lorente, Jose M. Palomo, Roberto Fernandez-Lafuente, and Jose M. Guisan Methods for Bioencapsulation of Enzymes and Cells Thomas M. S. Chang Index