内容説明
This text provides basic information on fluorescence spectroscopy, with a chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Each chapter is followed by a set of problems. The book also contains glossaries for commonly used acronyms and mathematical symbols, and for those wanting additional information the final appendix contains a list of recommended books which expand on various specialized topics.
目次
1. Introduction to Fluorescence. 2. Instrumentation for Fluorescence Spectroscopy. 3. Fluorophores. 4. Time-Domain Lifetime Measurements. 5. Frequency-Domain Lifetime Measurements. 6. Solvent Effects on Emission Spectra. 7. Dynamics of Solvent and Spectral Relaxation. 8. Quenching of Fluorescence. 9. Advanced Topics in Fluorescence Quenching. 10. Fluorescence Anisotropy. 11. Time-Dependent Anisotropy Decays. 12. Advanced Anisotropy Concepts. 13. Energy Transfer. 14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers. 15. Energy Transfer to Multiple Acceptors in One, Two or Three-Dimensions. 16. Protein Fluorescence. 17. Time-Resolved Protein Fluorescence. 18. Excited State Reactions. 19. Fluorescence Sensing. 20. Long Lifetime Metal-Ligand Complexes. 21. DNA Technology. 22. Phase Sensitive and Phase Resolved Emission Spectra. Appendixes: I. Corrected Emission Spectra. II. Fluorescent Lifetime Standards. III. Additional Reading Index
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