Protein expression : a practical approach

書誌事項

Protein expression : a practical approach

edited by S.J. Higgins and B.D. Hames

(The practical approach series, 202)

Oxford University Press, 1999

  • : hbk
  • : pbk

この図書・雑誌をさがす

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This text details the expression of cloned DNA or RNA templates in all the major "in vivo" and "in vitro" systems. The "in vivo" systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription translation. There is also a chapter on monitoring protein expression. This book is intended for any biomedical researcher wanting to investigate protein expression in cell free systems, viruses, prokaryotic cells, and eukaryotic cells.

目次

  • Protein expression in mammalian cells
  • expression in Xenopus oocytes and cell-free extracts
  • Expressing cloned genes in the yeasts Saccharomyces cerevisiae and Pichia pastoris
  • Baculovirus expression systems
  • protein synthesis in eukaryotic cell-free systems
  • Prokaryotic in vivo expression systems
  • cell-free coupled transcription-translation systems from Escherischia Coli
  • monitoring protein expression

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