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I observe the same behavior on Linux 4.18.0-17-generic #18-Ubuntu
with Python 3.6.7 (default, Oct 22 2018, 11:32:17) [GCC 8.2.0].
gdbm 1.14.1-6:
after
db = dbm.gnu.open("asdf","n")
db["a"] = "124"
db.reorganize()
db.close()
I see
$ lsof ~/asdf 
COMMAND PID USER FD TYPE DEVICE SIZE/OFF NODE NAME
python3 22071 sds mem-W REG 8,2 16384 4328326 /home/sds/asdf
and
>>> db = dbm.gnu.open("asdf","r")
Traceback (most recent call last):
 File "<stdin>", line 1, in <module>
 File "/home/sds/src/pyapp/tests/fileconv_test.py", line 1053, in <module>
_gdbm.error: [Errno 11] Resource temporarily unavailable