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MaxContrastProjection
This package is for version 3.6 of Bioconductor. This package has been removed from Bioconductor. For the last stable, up-to-date release version, see MaxContrastProjection.
Perform a maximum contrast projection of 3D images along the z-dimension into 2D
Bioconductor version: 3.6
A problem when recording 3D fluorescent microscopy images is how to properly present these results in 2D. Maximum intensity projections are a popular method to determine the focal plane of each pixel in the image. The problem with this approach, however, is that out-of-focus elements will still be visible, making edges and fine structures difficult to detect. This package aims to resolve this problem by using the contrast around a given pixel to determine the focal plane, allowing for a much cleaner structure detection than would be otherwise possible. For convenience, this package also contains functions to perform various other types of projections, including a maximum intensity projection.
Author: Jan Sauer, Bernd Fischer
Maintainer: Jan Sauer <jan.sauer at dkfz-heidelberg.de>
citation("MaxContrastProjection")):
Installation
To install this package, start R (version "3.4") and enter:
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("MaxContrastProjection")
For older versions of R, please refer to the appropriate Bioconductor release.
Documentation
To view documentation for the version of this package installed in your system, start R and enter:
browseVignettes("MaxContrastProjection")
Details
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Follow Installation instructions to use this package in your R session.