To install this package, start R and enter:
## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("ddCt")
In most cases, you don't need to download the package archive at all.
This package is for version 3.1 of Bioconductor; for the stable, up-to-date release version, see ddCt.
Bioconductor version: 3.1
The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.
Author: Jitao David Zhang, Rudolf Biczok and Markus Ruschhaupt
Maintainer: Jitao David Zhang <jitao_david.zhang at roche.com>
Citation (from within R,
enter citation("ddCt")):
To install this package, start R and enter:
## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("ddCt")
To view documentation for the version of this package installed in your system, start R and enter:
browseVignettes("ddCt")
Follow Installation instructions to use this package in your R session.
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