Bulked
segregant analysis (BSA) was employed for identifying putative markers associated with the head smut resistance.
Simple sequence repeat (SSR) markers were used in three different approaches to search for phytate loci in the Boggs-RR x CX1834-1-2 population: (i) bulked
segregant analysis (BSA; Michelmore et al., 1991), (ii) a modified BSA method which is described later, and (iii) a whole-genome scan of a subset of 94 random individuals.
Bulked
segregant analysis (Michelmore et al., 1991), simple F tests, and interval mapping (Lander and Botstein, 1989) are useful techniques for identifying molecular markers linked to a specific gene or region of the genome.
Abbreviations: AFLP, amplified fragment length polymorphism; BSA, bulked
segregant analysis; cM, centimorgans; FI, female index; LG, linkage group; LOD, likelihood of odds; PI, plant introduction; QTL, quantitative trait loci; SCN, soybean cyst nematode; SSR, simple sequence repeat.
The 256 AFLP primer pair combinations (EcoRI + ANN and MseI + CNN) were first tested on the parents and resistant and susceptible bulks developed using a combination of bulked
segregant analysis (BSA; Michelmore et al., 1991) and multiple-trait bulking procedure (Kolkman and Kelly, 2003) to detect associated markers.
Molecular mapping of a thermo-sensitive genetic male sterility gene in rice using bulked
segregant analysis.
The DNA marker for the velvet hairiness trait was identified by RAPD technique using bulked
segregant analysis.
The combined selective mapping strategy used to identify random amplified polymorphic DNA (RAPD) markers linked to the genomic regions conditioning resistance to Fusarium root rot consisted of bulked
segregant analysis (Michelmore et al., 1991) and selective genotyping (Lander and Botstein, 1989).
([paragraph]) Transgressive
segregant in the backcross generations.
Bulked
segregant analysis (BSA) (Michelmore et al., 1991) has made the process of identifying molecular markers linked with traits of interest more efficient; and it has been used successfully to detect molecular markers associated with disease resistance genes or quantitative trait loci (QTL) in many plant species, including soybean (Michelmore et al., 1991; Barua et al., 1993; Poulsen et al., 1995).
The inheritance of polymorphisms identified through bulked
segregant analyses was investigated utilizing 127 [F.sub.2] individuals in the Saluda x NCAG11 population.
Genetic mapping of Hessian fly avirulence gene vH6 using bulked
segregant analysis.