In order to meet the tissues' oxygen demand, most perfusion set-ups use gas-enriched
perfusates. A sufficient oxygen supply guarantees availability of ATP as an aerobic energy source, protecting and sustaining mitochondrial function.
DAmico et al., "The effect of normothermic machine perfusion (NMP) using various
perfusates on the hepatocellular and biliary epithelial integrity in porcine donation after cardiac death (DCD) models," Liver Transplantation, vol.
Surprisingly, salsolinol infusions had no effect on CRH concentration in
perfusates from the IN/ME.
Whiting, "Effect of mechanical stimulation on rabbits eyes: release of active substance in anterior chamber
perfusates," Journal of Physiology, vol.
The used
perfusates can vary according to the protocol used, for instance, minimal essential medium supplemented with human serum albumen or human tubal fluid medium supplemented with serum substitute supplement (SSS).
Insulin was measured in the
perfusates following the ramp studies, as well as in the lysates from freshly isolated islets by homogeneous time-resolved fluorescence technology (Cisbio Kit).
The RayBio[R] Human Fractalkine ELISA Kit (RayBiotech Inc., USA, catalogue number ELH-Fractalkine) applied in this study has a very high specificity that exceeds most other available ELISA tests for the detection of CX3CL1 in placental
perfusates, to the best of our knowledge.
Cammermeyer, "Argentophil neuronal perikarya and neurofibrils induced by postmortem trauma and hypertonic
perfusates," Acta Anatomica, vol.
FBAL (Fluoro Beta-Alanine) accumulated in cardiac tissue of rats for up to 8 days following a single dose.9 Using a fluorine-19 magnetic resonance imaging, Lemaire et al10 detected fluoroacetic acid in
perfusates of isolated rabbit\'s hearts and also observed cardiotoxicity and accumulation of citrate (presumably reflecting the inhibition of citrate metabolism).
Nitric oxide concentrations in the distal
perfusates from the intestinal test segment and in the plasma at the end of each experiment are found in Table 4.