The
Nucleolar Organizing Regions (NOR) staining revealed satellites on the long arm terminal region of chromosome 11 and not on 13p region.
The tumor
nucleolar grade was based on the International Society of Urologic Pathologists (ISUP) system, (22) and pathologic stage was based on the American Joint Committee on Cancer (AJCC) 8th edition.
Nucleophosmin,
nucleolar phosphoprotein B23, located within the nucleolus, is a nucleocytoplasmic shuttling protein involved in chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm as well as regulating the ARF-p53 tumor suppressor pathway.5 Mutations in the nucleophosmin (NPM1) gene are the most frequently occurring gene mutations in AML.6 These mutations identify patients that respond better to chemotherapy and have improved outcomes.7
Nucleolar protein 14 (NOP14) is a stress-response gene that encodes a
nucleolar protein required for the maturation of 18S rRNA and production of 40S ribosome (7).
RNU6 small
nucleolar RNA was quantified as internal control for data normalization.
Nucleolar organizer regions (AgNOR) and subepithelial vascularization as field cancerization markers in oral mucosa biopsies of alcoholic and smoking patients.
Involvement of human ribosomal proteins in
nucleolar structure and p53-dependent
nucleolar stress.
"A better understanding of how aspirin blocks TIF-IA and
nucleolar activity provides great promise for the development of new treatments and targeted therapy."
The nomenclature for nuclear patterns is primarily based on the reactivity observed in the nucleoplasm (for example, homogeneous or speckled) and the nuclear subcomponents that are recognized (for example, centromere or
nucleolar).
Therefore, when components for the assembly of large and small ribosomal subunits are stained with different fluorescents, the factors from different subunits should localize in distinct regions and the factors involved in the assembly of the same subunit should congregate together, indicating the presence of
nucleolar sub-compartments.