The slides were fixed and stained with ProLong[R] Gold Antifade
Mountant and DAPI (Thermo Fisher Scientific, USA), which stained the nuclei in blue, and were analyzed under a confocal microscope (DMI 6000; Leica, German).
Mount it with DPX
mountant and visualize under microscope.
The sections were then stained with standard Haematoxylin and Eosin method and then mounted (DPX
mountant, S.d.
They were then serially sectioned to 5 [micro]m thickness using microtomy, stained with Hematoxylin and Eosin (H&E) stain and were fixed on microscopic slides using DPX
mountant. The slides were then examined under a Motic DMB1 2MP light microscope (China) at 40X magnification for treatment-induced structural alterations and the photomicrographs were snapped.
The slides were visualized with diaminobenzine (DAB), counterstained by hematoxylin, dehydrated, and mounted in a neutral resin
mountant. Blue staining, as determination of TRIF protein expression, was displayed and examined using Nikon E800 microscope according to the manufacturer's instructions.
The cover slips were dried and placed on a labeled clean glass slides using a
mountant (DPX) for fluorescence microscopic examination (Leica Microsystems Limited, Heerbrugg, Switzerland) of VP2 antigen positive cells [28].
The segments were mounted with ProLong Gold antifade
Mountant reagent (Invitrogen, Carlsbad, CA, USA) on a glass slide.
After dehydration and clearing, sections were coverslipped with DPX
mountant (Sigma-Aldrich).