After 12 weeks, animals were anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) intraperitoneally, then rats were perfused
intracardially with 150 ml normal saline and 200 ml 4 % paraformaldehyde (PFA).
xylacine and ketamine, and
intracardially perfused with cold PBS.
Mice in the different groups were deeply anesthetized with chloral hydrate and perfused
intracardially with 30-40 ml of cold (4[degrees]C) heparinized 0.9% saline.
They were anesthetized with 70 mg/kg body weight ketamine and 7 mg/kg body weight xylazine and
intracardially perfused with 0.9% saline, followed by 4% paraformaldehyde (in phosphate buffer, pH 7.4), and then by the same fixative plus 10% sucrose.
After 2 h, the mice were
intracardially perfused with phosphate-buffered solution (PBS), and brain specimens were collected and rapidly weighed.
After the last behavioral session, rats were deeply anesthetized with an injection of sodium pentobarbital (120mg/kg, ip) and
intracardially perfused with PBS, followed by 4% paraformaldehyde.
Rats were perfused
intracardially with cold PBS containing heparin (0.2U/ml) and 4% paraformaldehyde and then stocked in 4% paraformaldehyde.
The remaining rats were anesthetized with 4% chloral hydrate and perfused
intracardially with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in PBS.
Blood samples were taken
intracardially to measure serum CK-MB levels.