I've had a ball with my own recently purchased
apochromat after using almost nothing but Schmidt-Cassegrain telescopes for many years.
Pictures of live HEK293 cells stably expressing [[beta].sub.2]AR-GFP were taken with an Axiovert[R] 200 M (Zeiss, Jena) fluorescence microscope equipped with argon laser, beam splitter HFT 488/543, oil immersion objective (Plan
Apochromat 63x/1.4), bandpass filter BP 505-530, and pinhole 96 mm was used.
In both study protocols, WSIs were created using a Mirax MIDI scanner (Zeiss, Oberkochen, Germany, currently sold as Pannoramic MIDI, PerkinElmer, Waltham, Massachusetts, produced by 3DHISTECH, Budapest, Hungary), which uses a X20 Zeiss 0.8 numerical aperture plan
apochromat objective, yielding images with 0.32 im/pixel.
There is a specially designed
Apochromat objective lens with high numerical aperture (0.11) and low distortion that provides crisp, clear images.
T3605) (nuclear staining) were acquired using Plan
Apochromat x 40/1.4 oil objective, 633 nm excitation wavelengths (helium-neon laser), and the 650-670 nm bandpass filter.
Superior optical performance is ensured through Nikon's new CFI Plan
Apochromat Lambda series objectives with Nano Crystal Coat technology.
Las celulas se fotografiaron con una camara digital (Nikon modelo Cool pix 5400) adaptada al microscopio, utilizando los objetivos Plan Neofluar 40X A.N 0,6 y Plan
Apochromat 100X A.N 1,4 (para inmersion en aceite).
* Apochromatic Lens: Lens in which red, blue and green colors are brought to a common focus by three lens components (also,
apochromat, ED or APO lens).