Detection of grain infestation caused by the granary weevil (Sitophilus granarius L.) using
zymography for [alpha]-amylase activity.
Gelatin
zymography assay was performed to evaluate the activity of MMP-2 and MMP-9 as previously described (12).
Zymography: Gelatin-specific
zymography technique was used for determination of MMPs activities in follicular fluid and seminal plasma.
[17] investigated enzyme activity by
zymography of MMPs-2, among others, in the serum of healthy men (n = 20), with BPH (n = 26), with localized PCa (n = 10), and with metastatic PCa (n = 15).
Gelatin Gel
Zymography. The supernatant of the cocultured microencapsulated cells was centrifuged at 2000 rpm for 10 min; next, it was frozen in -80[degrees]C.
Noteworthy, we analyzed MMP-9 levels using an ELISA technique, in contrast to many previous studies on MMP-9, which determined its levels using a
zymography technique [11].
Gelatin
Zymography. MMP-2/collagenases activity was assessed by gelatin
zymography by using conditioned medium obtained from BM-MSCs essentially as previously reported [17].
Li, "Reveal the response of enzyme activities to heavy metals through in situ
zymography," Ecotoxicology and Environmental Safety, vol.
Gelatine
Zymography. In order to confirm the character of oviductal MMPs, OF was incubated in the presence of APMA (aminophenylmercuric acetate) to activate the latent zymogen and induce the transition of proMMP to MMP, and analysed by
zymography.
We will investigate effects on cell proliferation (DNA assay), elastic matrix deposition (Fastin assay), fiber formation and crosslinking (desmosine assay), and elastic matrix breakdown processes mediated by matrix metalloproteases (MMPs) (western blot, gel
zymography).