Mustard also contains a glucosinolate called
sinigrin. The combination of myronsinase and
sinigrin produces AITC, which is responsible for the characteristic pungency of mustard oil.
In addition some studies have identified effective compounds within Erysimum plants such as methylthioalkyl, methylsulfinylalkyl and methylsulfonylalkyl glucosinolates [19],
sinigrin, progoitrin, glucoiberin, 3-(methylcarbonyl)propyl glucosinolate, glucocheirolin and glucoerysolin [20], 4-isothiocyanatobutanoic acid [21], cardiac glycoside [22].
Glucosinolates (progoitrin,
sinigrin, and gluconapin) were confirmed by a Finnigan LCQ Deca XP plus Ion Trap Mass Spectrometer system (Thermo Finnigan, San Jose, CA, USA) which confirmed by LC-ESI-MS in positive mode.
xylostella has been investigated based on two major characteristics: the presence of alkanes in the foliar wax, and
sinigrin content in leaves (Lin et al.
pekinensis, (ii) the GL standard of
sinigrin (Sigma-Aldrich, Taufkirchen, Germany), and (iii) Gl standard of glucotropaeolin (benzyl GL) (ceded by Professor of Eduardo Rosa from University of Tras-os-Montes e Alto Douro, Vila Real, Portugal and Dr.
Glucoiberin, progoitrin, glucoraphanin,
sinigrin, gluconapin, and glucoerucin were detected as aliphatic glucosinolates as well as an aromatic glucosinolate gluconasturtiin.
For example, Syringinenin (MOL000347, DL=0.32, 0B=14.6%),
Sinigrin (MOL013312, DL=0.18, OB=3.9%), Goitrin (MOL001811, DL=0.01, OB=3.2%), Sinigrin_qt (MOL001761, DL=0.03, OB=21.2%) being the major bioactive ingredients of Radix Isatidis.
Spodoptera litura was reared on 2 diets, i.e., on castor leaves (Ricinus communis L.; Malpighiales: Euphorbiaceae) which is the natural food, and a synthetic meridic diet containing chickpea seeds and
sinigrin as a phagostimulant.
Developmental changes of
sinigrin and glucoraphanin in three Brassica species (Brassica nigra, Brassica juncea and Brassica oleracea var.