In the present study, there were significant differences in the treatment groups in [alpha] linolenic acid, linolenic acid, palmitoleic acid, arachidonic acid,
lignoceric acid and DHA (p<0.05).
SFAs were detected as the sum of palmitic acid (C16:0), margaric acid (C17:0), stearic acid (C18:0), arachidic acid (C20:0), and
lignoceric acid (C24:0).
Palmitic C 16 : 0 8.54 [+ or -] 0.03 Palmitoleic C 16 : 1 0.00 [+ or -] 0.00 (A) Stearic C 18 : 0 6.83 [+ or -] 0.01 Oleic C 18 : 1(n-9) 34.12 [+ or -] 0.09 Vaccenic C 18 : 1(n-7) 0.00 [+ or -] 0.00 (B) Linoleic C 18 : 2(n-6) 48.86 [+ or -] 0.08 Arachidic C 20 : 0 0.87 [+ or -] 0.03 (C) Eicosenoic C 20 : 1 0.18 [+ or -] 0.01 Linolenic C 18 : 3(n-3) 0.41 [+ or -] 0.00 Docosanoic C 22 : 0 0.19 [+ or -] 0.01 (D) Erucic C 22 : 1 0.00 [+ or -] 0.00
Lignoceric C 24 : 0 0.00 [+ or -] 0.00 (E) Nervonic C 24 : 1 0.00 [+ or -] 0.00 (F) [summation] saturated -- 16.43 [+ or -] 0.02 [summation] monounsaturated -- 34.30 [+ or -] 0.09 [summation] polyunsaturated -- 49.27 [+ or -] 0.08 Fatty acids (%) R.
of carbon atoms Systematic name and double bonds Adansonia digitata C14:0 Myri stic 0.2 [+ or -] 0.0 C16:0 Palmitic 25.7 [+ or -] 0.2 C16:1 Palmitoleic 0.2 [+ or -] 0.0 C17:0 Margaric 0.2 [+ or -] 0.0 C17:1 Margaroleic 0.2 [+ or -] 0.0 C18:0 Stearic 4.6 [+ or -] 0.1 C18:1 Oleic 34.9 [+ or -] 0.7 C18:2 Linoleic (2) 29.9 [+ or -] 0.9 C18:3 Linolenic (2) 2.1 [+ or -] 0.0 C20:0 Arachidic 0.9 [+ or -] 0.1 C20:1 Eicosenoic 0.2 [+ or -] 0.0 C22:0 Behenic 0.3 [+ or -] 0.0 C22:1 Erucic 0.1 [+ or -] 0.0 C24:0
Lignoceric 0.2 [+ or -] 0.0 Fatty acid fat [+ or -] SD No.
In fact, the postprandial serum contained few amount of some long chain fatty acids, docosatetraenoic (C22:4),
lignoceric (C24:0), and nervonic (C24:1) acids, which were not detected in fasting serum (Supplementary Table 1).
According to ANOVA analysis, the amount of [omega]-9 palmitoleic acid (C16:1[omega]-9), [omega]-7 and [omega]-11 oleic acids (C18:1[omega]-7, C18:1[omega]-11), behenic acid (C22:0), and
lignoceric acid (C24:0) did not differ statistically among the samples (p > 0.05), whereas for all other fatty acids a statistical difference was found (p [less than or equal to] 0.05) for this set of samples.
Twenty-four fractions of FFAs (lauric, myristic, myristoleic, palmitic, palmitoleic, stearic, oleic, linoleic, gamma-linolenic, linolenic, arachidic, eicosenoic, eicosadienoic, 5-8-11-eicosatrienoic, dihomo-gamma-linolenic, arachidonic, eicosapentaenoic, behenic, erucic, docosatetraenoic, docosapentaenoic,
lignoceric, docosahexaenoic, and nervonic acid) (Table 2) were determined by capillary gas chromatography (HP 6890, Hitachi, 30 m capillary column, inner diameter 0.32mm, phase layer 0.25fm) [14].
FL,
lignoceric acid, palmitic acid, boric acid, potassium chloride, sodium hydroxide, chloroform, methanol, and ethanol were purchased from Wako Pure Chemicals Industries, Ltd.
Additionally, tetracosanoic (
lignoceric, 24: 0), docosanoic (behenic, 22: 0), eicosenoic (gadoleic, 20 : 1), and eicosanoic (arachidic, 20 : 0) acids were detected at low concentrations.