Heterogenetic

Mouse strains are divided into lineages that result from mating between siblings (inbred) called isogenic, while colonies from random mating (outbred) are said to be heterogenetic. Each inbred line has a unique gene set, since the consanguineous mating decreases the frequency of heterozygous genotypes, generating a homoallelic state, that is, where there is only one allelic variant present that leads to the fixation of the alleles of that group.

Only heterogenetic mice (GFP/+) were used for experiments.

For immunohistochemistry tests, antigen retrieval was conducted and the endogenous peroxidase and heterogenetic antigen were blocked by 3% [H.sub.2][O.sub.2] and 10% goat serum, respectively.

The reason may be the heterogenetic of coal samples.