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Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes

Nature volume 348, pages 510–514 (1990)Cite this article

Abstract

A complementary DNA encoding a voltage-gated chloride channel from Torpedo marmorata electric organ was cloned by expressing hybrid-depleted messenger RNA in Xenopus oocytes. The predicted protein has a sequence of 805 amino acids containing several putative membrane-spanning domains. Expression of the protein in Xenopus oocytes shows that it is sufficient for channel function.

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References

  1. Worrell, R. T., Butt, A. G., Cliff, W. H. & Frizzell, R. A. Am. J. Physiol. 256, C1111–C1119 (1989).

    Article CAS Google Scholar

  2. Gögelein, H. Biochim. biophys. Acta 947, 521–547 (1988).

    Article Google Scholar

  3. Liedtke, C. M. A. Rev. Physiol. 51, 143–160 (1989).

    Article CAS Google Scholar

  4. Bretag, A. H. Physiol. Rev. 67, 618–724 (1987).

    Article CAS Google Scholar

  5. Frizzel, R. A. Trends Neurosci. 10, 190–193 (1987).

    Article Google Scholar

  6. Riordan, J. R. et al. Science 245, 1066–1073 (1989).

    Article ADS CAS Google Scholar

  7. Welsh, M. J. FASEB J. 4, 2718–2725 (1990).

    Article CAS Google Scholar

  8. Rüdel, R. & Lehmann-Horn, F. Physiol. Rev. 65, 310–356 (1985).

    Article Google Scholar

  9. Schofield, P. R. et al. Nature 328, 221–227 (1987).

    Article ADS CAS Google Scholar

  10. Grenningloh, G. et al. Nature 328, 215–220 (1987).

    Article ADS CAS Google Scholar

  11. Landry, D. W. et al. Science 244, 1469–1472 (1989).

    Article ADS CAS Google Scholar

  12. Finn, A. L., Tsai, L.-M., Falk, R. J. Proc. natn. Acad. Sci. U.S.A. 86, 7649–7652 (1989).

    Article ADS CAS Google Scholar

  13. White, M. M. & Miller, C. J. biol. Chem. 254, 10161–10166 (1979).

    CAS PubMed Google Scholar

  14. Miller, C. & White, M. M. Proc. natn. Acad. Sci. U.S.A. 81, 2772–2775 (1984).

    Article ADS CAS Google Scholar

  15. Miller, C. & Richard, E. A. in Chloride Channels and Carriers in Nerve, Muscle and Glial Cells (eds Alvarez-Leefsmans, F. J., & Russel, J. M.) 383–405 (Plenum, New York 1990).

    Book Google Scholar

  16. Taguchi, T. & Kasai, M. Biochem. biophys. Res. Commun. 96, 1088–1094 (1980).

    Article CAS Google Scholar

  17. Jentsch, T. J., Garcia, A. M. & Lodish, H. F. Biochem. J. 261, 155–166 (1989).

    Article CAS Google Scholar

  18. Kroll, B. et al. Am. J. Physiol. 257, L284–L288 (1989).

    CAS PubMed Google Scholar

  19. Sumikawa, K., Parker, I. & Miledi, R. EMBO J. 3, 2291–2294 (1984).

    Article CAS Google Scholar

  20. Barish, M. E. J. Physiol., Lond. 342, 309–325 (1983).

    Article ADS CAS Google Scholar

  21. Boton, R., Singer, D., & Dascal, N. Pflügers Arch. Eur. J. Physiol. 416, 1–6 (1990).

    Article CAS Google Scholar

  22. Hediger, M., Coady, M. J., Ikeda, T. S. & Wright, E. M. Nature 330, 379–381 (1987).

    Article ADS CAS Google Scholar

  23. Masu, Y. et al. Nature 329, 836–838 (1987).

    Article ADS CAS Google Scholar

  24. Lübbert, H. et al. Proc. natn. Acad. Sci. U.S.A. 84, 4332–4336 (1987).

    Article ADS Google Scholar

  25. Vieira, J. & Messing, J. Meth. Enzym. 153, 3–11 (1987).

    Article CAS Google Scholar

  26. Kawasaki, E. S. Nucleic Acids Res. 13, 4991–5004 (1985).

    Article CAS Google Scholar

  27. Distefano, A. et al. Pflügers Arch. Eur. J. Physiol. 405, S95–S100 (1985).

    Article CAS Google Scholar

  28. Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).

    Article CAS Google Scholar

  29. Lipman, D. J. & Pearson, W. R. Science 227, 1435–1441 (1985).

    Article ADS CAS Google Scholar

  30. Chou, P. Y. & Fasman, G. D. A. Rev. Biochem. 47, 251–276 (1978).

    Article CAS Google Scholar

  31. Edelman, A. M., Blumenthal, D. K. & Krebs, E. G. A. Rev. Biochem. 56, 567–613 (1987).

    Article CAS Google Scholar

  32. Staden, R. Nucleic Acids Res. 10, 2951–2961 (1982).

    Article CAS Google Scholar

  33. Papazian, D. M., Schwarz, T. L., Tempel, B. L., Jan, Y. N. & Jan, L. Y. Science 237, 749–753 (1987).

    Article ADS CAS Google Scholar

  34. Baumann, A. et al. EMBO J. 6, 3419–3429 (1987).

    Article CAS Google Scholar

  35. Kamb, A., Iverson, L. E. J. & Tanouye, M. A. Cell 50, 405–413 (1987).

    Article CAS Google Scholar

  36. Noda, M. et al. Nature 312, 121–127 (1984).

    Article ADS CAS Google Scholar

  37. Tanabe, T. et al. Nature 328, 313–318 (1987).

    Article ADS CAS Google Scholar

  38. Kaupp, U. B. et al. Nature 342, 762–766 (1989).

    Article ADS CAS Google Scholar

  39. Jan, L. Y. & Jan, Y. N. Nature 345, 672 (1990).

    Article ADS CAS Google Scholar

  40. Takumi, T., Ohkubo, H. & Nakanishi, S. Science 242, 1042–1045 (1988).

    Article ADS CAS Google Scholar

  41. Sansom, S. C., La, B.-Q. & Carosi, S. L. Am. J. Physiol. 259, F46–F52 (1990).

    CAS PubMed Google Scholar

  42. Colman, A. in Translation and Transcription (eds Hames, B. D. & Higgins, S. J.) 271–302 (IRL, Oxford, 1984).

    Google Scholar

  43. Jentsch, T. J., Matthes, H., Keller, S. K. & Wiederholt, M. Pflügers Arch. Eur. J. Physiol. 403, 175–185 (1985).

    Article CAS Google Scholar

  44. Gubler, U. & Hofman, B. J. Gene 25, 263–269 (1983).

    Article CAS Google Scholar

  45. Seed, B. Nature 329, 840–842 (1987).

    Article ADS CAS Google Scholar

  46. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article ADS CAS Google Scholar

  47. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article CAS Google Scholar

  48. Kopito, R. R. & Lodish, H. F. Nature 316, 234–238 (1985).

    Article ADS CAS Google Scholar

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Authors and Affiliations

  1. Centre for Molecular Neurobiology (ZMNH), Hamburg University, Martinistrasse 52, D-2000, Hamburg, 20, Germany

    Thomas J. Jentsch, Klaus Steinmeyer & Gisela Schwarz

Authors
  1. Thomas J. Jentsch

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  2. Klaus Steinmeyer

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  3. Gisela Schwarz

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Jentsch, T., Steinmeyer, K. & Schwarz, G. Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes. Nature 348, 510–514 (1990). https://doi.org/10.1038/348510a0

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  • DOI: https://doi.org/10.1038/348510a0

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