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9780121820855

Avidin-Biotin Technology

by ; ; ;
  • ISBN13:

    9780121820855

  • ISBN10:

    0121820858

  • Format: Hardcover
  • Copyright: 1990年05月28日
  • Publisher: Elsevier Science

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Summary

The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.

Table of Contents

Introduction
Reflections
Introduction to Avidin-Biotin Technology
Applications of Avidin-Biotin Technology: Literature Survey
Biotin-Binding Proteins
Biotin-Binding Proteins: Overview and Prospects
Avidin and Streptavidin
Nonglycosylated Avidin
Cloning and Expression of Avidin in Escherichia coli
Isolation and Properties of Streptavidin
Crystal Forms of Avidin
Non-Avidin Biotin-Binding Proteins
Biotinidase
Monoclonal Antibody to Biotin
General Methodology: Preparation of Biotin Derivatives
Biotin-Containing Reagents
Protein Biotinylation
Enzymatic C-Terminal Biotinylation of Proteins
Antibodies Biotinylated via Sugar Residues
2-Iminobiotin-Containing Reagent and Affinity Columns
Preparation of Avidin and Streptavidin Derivatives
Avidin- and Streptavidin-Containing Probes
Phycobiliprotein-Avidin and Phycobiliprotein-Biotin Conjugates
Monovalent Avidin Affinity Columns
Prevention of Nonspecific Binding of Avidin
Assays for Avidin and Biotin
Enzymatic and Radioactive Assays for Biotin, Avidin, and Streptavidin
Colorimetric Enzyme Assays for Avidin and Biotin
Sequential Solid-Phase Assay for Biotin Based on 125I-Labeled Avidin
Fluorometric Assay for Avidin-Biotin Interaction
Applications: Isolation and Purification
Isolation of Biologically Active Compounds: A Universal Approach
Isolation and Characteriszation of Hormone Receptors
Biotinylation of Peptide Hormones: Structural Analysis and Application to Flow Cytometry
Purification of Gonadotropin-Releasing Hormone
Biotinylestradiol for Purfication of Estrogen Receptor
One-Step Immunoaffinity Purification of Transferrin
Lectin-Mediated Isolation of Cell Surface Glyoproteins
Immunoselective Cell Separation
Isolation and Analysis of Splicing Complexes
Z-DNA Affinity Chromatography
Localization
Affinity Cytochemistry
Double Fluorescence Immunolabeling Studies
Table of Contents provided by Publisher. All Rights Reserved.

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