タンパク質結晶化構造解析から宇宙実験まで:コンフォーカルサイエンス

■しかく技術情報

■しかく製品説明書

C-Kit Ground Pro XRD: X線回折実験向けのタンパク質結晶作成研究用キットです。>>C-Kit Ground Pro XRDの取扱説明書はこちら
C-Kit Ground Pro ND: 中性子線回折実験向けのタンパク質結晶作成研究用キットです。>>C-Kit Ground Pro NDの取扱説明書はこちら

■しかく実験操作動画

■しかく 1.結晶化試薬の調製

結晶化試薬の調製の説明動画です

■しかく 2.タンパク質溶液充填

タンパク質溶液充填の説明動画です

■しかく 3.シーリングコンパウンド

シーリングコンパウンドの説明動画です

■しかく 4.ゲルチューブ

ゲルチューブの説明動画です

■しかく C-Kit Education

C-Kit Educationの実験操作の説明動画です

■しかく C-Tube LCDM

C-Tube LCDMの充填方法の説明動画です

■しかくバッチ法

ガラスキャピラリーを利用したバッチ法の充填方法の説明動画です

■しかく外部発表リスト

■しかく宇宙実験:微小重力効果

宇宙環境を利用したタンパク質結晶化実験の技術的背景 Background of protein crystallization technologies in space. 高橋幸子, 田仲広明. Int. J. Microgravity Sci. Appl. (2017). 34(1), 340103.(Japanese)
https://doi.org/10.15011//jasma.34.340103
宇宙ならびに地上で生成したタンパク質結晶のX線回折能の不均一性 Non-uniform diffractive quality of the crystals grown in microgravity and in terrestrial gravity. 高橋幸子, 中川敦史, 古林直樹, 伊中浩治, 太田和敬, 田仲広明. 日本結晶成長学会誌 (2016). 43(2), 47–53.(Japanese)
https://doi.org/10.19009/jjacg.43.2_110
Numerical model of protein crystal growth in a diffusive field such as the microgravity environment. H. Tanaka, S. Sasaki, S. Takahashi, K. Inaka, Y. Wada, M. Yamada, K. Ohta, H. Miyoshi, T. Kobayashi and S. Kamigaichi, J. Synchrotron Rad. (2013). 20, 1003–1009.
https://doi.org/10.1107/S0909049513022784
Numerical analysis of the diffusive field around a growing protein crystal in microgravity. K. Inaka, H. Tanaka, S. Takahashi, S. Sano, M. Sato, M. Shirakawa and Y. Yoshimura. Defect and Diffusion Forum Vols. 323-325 (2012). pp565-569.
https://doi.org/10.4028/www.scientific.net/ddf.323-325.565
Controlling the Diffusive Field to Grow a Higher Quality Protein Crystal in Microgravity. H. Tanaka, K. Inaka, N. Furubayashi, M. Yamanaka, S. Takahashi, S. Sano, M. Sato, M. Shirakawa and Y. Yoshimura, Defect and Diffusion Forum Vols. 323-325 (2012). pp549-554.
https://doi.org/10.4028/www.scientific.net/ddf.323-325.549
宇宙実験での蛋白質結晶の高品質化事前予測技術. 田仲広明、伊中浩治、古林直樹、佐藤勝、高橋幸子、山中麻里、広田恵理華、佐野智、小林智之、田中哲夫日本マイクログラビティ応用学会誌 (2008). 25(2),101-106 (Japanese).
https://doi.org/10.15011/jasma.25.2.101
宇宙環境を利用した高品質なタンパク質結晶の生成. 高橋幸子、田仲広明, 日本結晶学会誌(2004). 46, 323-331 (Japanese).
https://doi.org/10.5940/jcrsj.46.323
Numerical Analysis of the Depletion Zone Formation Around a Growing Protein Crystal. Hiroaki Tanaka, Koji Inaka, Shigeru Sugiyama, Sachiko Takahashi, Satoshi Sano, Masaru Sato, Susumu Yoshitomi. Transport Phenomena in Microgravity (2004), 1027(1), 10-19.
https://doi.org/10.1196/annals.1324.002

■しかく宇宙実験技術:容器

A Small Device for Two-step Enzymatic Reactions in Space. Sachiko TAKAHASHI, Tomohiro KUGA, Misako KOGA, Bin YAN, Naoki SUNAGAWA, Akiko TANAKA, Kiyohito IGARASHI and Hiroaki TANAKA. Int. J. Microgravity Sci. Appl. (2024). 41(4), 410401.
https://doi.org/10.15011//jasma.41.410401

JCB-SGT Crystallization Devices Applicable to PCG Experiments and their Crystallization Conditions. Sachiko TAKAHASHI, Misako KOGA, Bin YAN, Naoki FURUBAYASHI, Masayuki KAMO, Koji INAKA and Hiroaki TANAKA. Int. J. Microgravity Sci. Appl. (2019). 36(1), 360107.
https://doi.org/10.15011//jasma.36.360107

■しかく宇宙実験技術:実験プロジェクト

構造解析向けタンパク質結晶化宇宙実験に必要な実験技術 Essential Techniques for growing high-quality protein crystals for structural analysis in microgravity. 伊中浩治, 高橋幸子, 田仲広明. Int. J. Microgravity Sci. Appl. (2017). 34(1), 340104.(Japanese)
https://doi.org/10.15011//jasma.34.340104
JAXA protein crystallization in space: ongoing improvements for growing high-quality crystals. S. Takahashi, K. Ohta, N. Furubayashi, B. Yan, M. Koga, Y. Wada, M. Yamada, K. Inaka, H. Tanaka, H. Miyoshi, T. Kobayashi and S. Kamigaichi, J. Synchrotron Rad. (2013). 20, 968–97.
https://doi.org/10.1107/S0909049513021596
「きぼう」を利用した高品質タンパク質結晶生成実験の現状と合理的な宇宙実験へのアプローチ. 高橋幸子、厳斌、古林直樹、正木美佳、太田和敬、伊中浩治、田仲広明、小林智之、吉村善範. 日本マイクログラビティ応用学会誌 (2012).29(3),111-119.
https://doi.org/10.15011/jasma.29.3.111
国際宇宙ステーションを利用した高品質タンパク質結晶生成実験技術のスピンオフ. 田仲広明、高橋幸子、古林直樹、山中麻里、厳斌、広田恵理華、佐野智、佐藤勝、伊中浩治、小林智之、田中哲夫.日本マイクログラビティ応用学会誌 (2010). 27(2), 104-112.
https://doi.org/10.15011/jasma.27.2.104
JAXA-GCF project - High-quality protein crystals grown under microgravity environment for better understanding of protein structure. Sato, M., Tanaka, H., Inaka, K. et al. Microgravity Sci. Technol 18, 184–189 (2006).
https://doi.org/10.1007/BF02870406

■しかく宇宙実験技術:成果

Bacterial Cellulose Production in Space. Akiko Tanaka, Tomoya Imai, Misako Koga, Naoki Sunagawa, Kiyohiko Igarashi, Kenji Tajima, Hiroaki Tanaka. Int. J. Microgravity Sci. Appl. 41(3) (2024) 410302.
https://doi.org/10.15011/jasma.41.410302
Effect of Impurity Concentration on the Precipitation of Atorvastatin Calcium Under Microgravity on the International Space Station. Makoto Otsuka, Yota Kuroyanagi, Tetsuo Sasaki, Masakazu Matsumoto, Yutaro Hirose, Hiroaki Tanaka and Naohiro Sato. Colloids and Surfaces A: Physicochemical and Engineering Aspects (2024) 134553.
https://doi.org/10.1016/j.colsurfa.2024.134553
Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase. Junko Okuda-Shimazaki, Hiromi Yoshida, Inyoung Lee, Katsuhiro Kojima, Nanoha Suzuki, Wakako Tsugawa, Mitsugu Yamada, Koji Inaka, Hiroaki Tanaka and Koji Sode. Communications Biology 5 (2022) 1334.
https://doi.org/10.1038/s42003-022-04286-9
Fragment-based discovery of the first nonpeptidyl inhibitor of an S46 family peptidase. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Akihiro Nakamura, Yurie Watanabe, Mizuki Sekiya, Saori Roppongi, Chisato Kushibiki, Ippei Iizuka, Osamu Tani, Hitoshi Sakashita, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta, Nobuyuki Honma, Yosuke Shida, Wataru Ogasawara, Mayumi Nakanishi-Matsui, Takamasa Nonaka, Hiroaki Gouda & Nobutada Tanaka. Scientific Reports volume 9, Article number: 13587 (2019).
https://doi.org/10.1038/s41598-019-49984-3
Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery. Itoh T, Hibi T, Suzuki F, Sugimoto I, Fujiwara A, et al. PLOS ONE (2016) 11(12): e0167310.
https://doi.org/10.1371/journal.pone.0167310
"Newton’s cradle" proton relay with amide–imidic acid tautomerization in inverting cellulase visualized by neutron crystallography. Akihiko Nakamura, Takuya Ishida, Katsuhiro Kusaka, Taro Yamada, Shinya Fushinobu, Ichiro Tanaka, Satoshi Kaneko, Kazunori Ohta, Hiroaki Tanaka, Koji Inaka, Yoshiki Higuchi, Nobuo Niimura, Masahiro Samejima and Kiyohiko Igarashi. SCIENCE ADVANCES21 AUG 2015 : E1500263.
https://doi.org/10.1038/srep11151
Structural and mutational analyses of dipeptidyl peptidase 11 from Porphyromonas gingivalis reveal the molecular basis for strict substrate specificity. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta, Hiroaki Gouda, Takamasa Nonaka, Wataru Ogasawara & Nobutada Tanaka. Scientific Reports volume 5, Article number: 11151 (2015).
https://doi.org/10.1038/srep11151
S46 Peptidases are the First Exopeptidases to be Members of Clan PA. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mika Masaki, Kazunori Ohta, Hirofumi Okada, Takamasa Nonaka, Yasushi Morikawa, Kazuo T. Nakamura, Wataru Ogasawara & Nobutada Tanaka . Sci Rep 4, 4977 (2014).
https://doi.org/10.1038/srep04977
High-Quality Protein Crystal Growth of Mouse Lipocalin- Type Prostaglandin D Synthase in Microgravity. K. Inaka, S. Takahashi, K. Aritake, T. Tsurumura, N. Furubayashi, B. Yan, E. Hirota, S. Sano, M. Sato, T. Kobayashi, Y. Yoshimura, H. Tanaka and Y. Urade, Cryst Growth Des. (2011). June 1; 11(6): 2107-2111.
https://doi.org/10.1021/cg101370v
Improvement in the quality of hematopoietic prostaglandin D synthase crystals in a microgravity environment. H. Tanaka, T. Tsurumura, K. Aritake, N. Furubayashi, S. Takahashi, M. Yamanaka, E. Hirota, S. Sano, M. Sato, T. Kobayashi, T. Tanaka, K. Inaka and Y. Urade. J. Synchrotron Rad. (2011). 18, 88-91.
https://doi.org/10.1107/S0909049510037076
High-quality crystals of human haematopoietic prostaglandin D synthase with novel inhibitors. S. Takahashi, T. Tsurumura, K. Aritake, N. Furubayashi, M. Sato, M. Yamanaka, E. Hirota, S. Sano, T. Kobayashi, T. Tanaka, K. Inaka, H. Tanaka and Y. Urade. Acta Cryst. (2010). F66, 846-850.
https://doi.org/10.1107/S1744309110020828
ISSを利用した高品質タンパク質結晶生成実験の応用利用. 高橋幸子、佐野智、佐藤勝、広田恵理華、古林直樹、厳斌、小林智之、伊中浩治、田仲広明、田中哲夫日本マイクログラビティ応用学会誌 (2010). 27(2), 98-103.
https://doi.org/10.15011/jasma.27.2.98
宇宙実験による高分解能タンパク質構造解析の応用に向けて. 高橋幸子、古林直樹、鶴村俊治、有竹浩介、佐藤勝、山中麻里、広田恵理華、佐野智、伊中浩治、田仲広明、小林智之、裏出良博、田中哲夫.日本マイクログラビティ応用学会誌 (2008).25(2),141-146.
https://doi.org/10.15011/jasma.25.2.141
Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions. H. Tanaka, T. Umehara, K. Inaka, S. Takahashi, R. Shibata, Y. Bessho, M. Sato, S. Sugiyama, E. Fusatomi, T. Terada, M. Shirouzu, S. Sano, M. Motohara, T. Kobayashi, T. Tanaka, A. Tanaka and S. Yokoyama. Acta Cryst. (2007). F63, 69-73.
https://doi.org/10.1107/S1744309106054625

■しかく結晶化関連技術

Through Diffusion Measurements of Molecules to a Numerical Model for Protein Crystallization in Viscous Polyethylene Glycol Solution. Hiroaki Tanaka, Rei Utata, Keiko Tsuganezawa, Sachiko Takahashi and Akiko Tanaka. Crystals (2022). 12, 881.
https://doi.org/10.3390/cryst12070881
Novel Device and Strategy for Growing Large, High-Quality Protein Crystals by Controlling Crystallization Conditions. Naoki Tanigawa, Sachiko Takahashi, Bin Yan, Masayuki Kamo, Naoki Furubayashi, Koji Kubota, Koji Inaka and Hiroaki Tanaka. Crystals (2021). 11, 1311.
https://doi.org/10.3390/cryst11111311
Semi-empirical model to estimate ideal conditions for the growth of large protein crystals. Hirohiko Nakamura, Sachiko Takahashi, Koji Inaka and Hiroaki Tanaka. Acta Cryst. D. 76 (2020) 1174-1183.
https://doi.org/10.1107/S205979832001445X
Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization. Yoshinobu. Hashizume, Koji Inaka, Naoki Furubayashi, Masayuki Kamo, Sachiko Takahashi and Hiroaki Tanaka. Crystals (2020). 10, 78.
https://doi.org/10.3390/cryst10020078
A novel handling-free method of mounting single protein crystals for synchrotron structure analyses at room temperature. Yoshihisa Suzuki, Kumi Torii, Koji Inaka, Takahisa Fujiwara, Hiroaki Tanaka and Yoshitomo Arai. Review of Scientific Instruments 90, 054101 (2019).
https://doi.org/10.1063/1.5070122
Optimization of salt concentration in PEG-based crystallization solutions. M. Yamanaka, K. Inaka, N. Furubayashi, M. Matsushima, S. Takahashi, H. Tanaka, S. Sano, M. Sato, T. Kobayashi and T. Tanaka. J. Synchrotron Rad. (2011). 18, 84-87.
https://doi.org/10.1107/S0909049510035995
Diffusion coefficient of the protein in various crystallization solutions: The key to growing high-quality crystals in space. Hiroaki Tanaka, Sachiko Takahashi, Mari Yamanaka, Izumi Yoshizaki, Masaru Sato, Satoshi Sano, Moritoshi Motohara, Tomoyuki Kobayashi, Susumu Yoshitomi, Tetsuo Tanaka and Seijiro Fukuyama. Microgravity Sci. Technol 18, 91–94 (2006).
https://doi.org/10.1007/BF02870387
A simplified counter diffusion method combined with a 1D simulation program for optimizing crystallization conditions. H. Tanaka, K. Inaka, S. Sugiyama, S. Takahashi, S. Sano, M. Sato and S. Yoshitomi. J. Synchrotron Rad. (2004). 11, 45-48.
https://doi.org/10.1107/S0909049503023446

■しかく SETM教育

日本の宇宙環境利用とSTEM教育. 田仲広明. レデックスメルマガ. 2024年1月19日
国際宇宙ステーションを使ったSTEM教育への動機づけ. 田仲広明. レデックスメルマガ. 2024年1月5日

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■しかく 技術情報

■しかく 製品説明書

■しかく 実験操作動画

■しかく 1.結晶化試薬の調製

■しかく 2.タンパク質溶液充填

■しかく 3.シーリングコンパウンド

■しかく 4.ゲルチューブ

■しかく C-Kit Education

■しかく C-Tube LCDM

■しかく バッチ法

■しかく 外部発表リスト

■しかく 宇宙実験:微小重力効果

■しかく 宇宙実験技術:容器

■しかく 宇宙実験技術:実験プロジェクト

■しかく 宇宙実験技術:成果

■しかく 結晶化関連技術

■しかく SETM教育

■しかく技術情報

■しかく製品説明書

■しかく実験操作動画

■しかくバッチ法

■しかく外部発表リスト

■しかく宇宙実験:微小重力効果

■しかく宇宙実験技術:容器

■しかく宇宙実験技術:実験プロジェクト

■しかく宇宙実験技術:成果

■しかく結晶化関連技術