Description:
Multisite phosphorylation of S46 and T55 in the PH-like binding motif of Cellular tumor antigen p53 (TP53) gradually enhances its affinity for General transcription factor IIH subunit 1 (GTF2H1), an interaction involved in activation of transcription initiation and elongation by Cellular tumor antigen p53 (TP53).
Participants:
(1) Cellular tumor antigen p53 (TP53)
(2) General transcription factor IIH subunit 1 (GTF2H1)
Interactions
Interaction #1 TP53 -
GTF2H1 Interfaces (1) ELM:LIG_PH_Tfb1 motif (
50IEQWFTE56) in
Cellular tumor antigen p53 (TP53) (2) TFIIH p62 subunit, N-terminal domain (9-81) in
General transcription factor IIH subunit 1 (GTF2H1) Interaction Regulation PTM-dependent Enhancement (Phosphorylation of
S46 and
T55 on
Cellular tumor antigen p53 (TP53)) of the
Cellular tumor antigen p53 (TP53) LIG_PH_Tfb1 motif -
General transcription factor IIH subunit 1 (GTF2H1) TFIIH p62 subunit, N-terminal domain interaction
Inferred Regulatory Enzymes for switch Putative modifying enzymes for residue:
S46 : Serine-protein kinase ATM (ATM), ATM,
Cyclin-dependent kinase 5 (CDK5),
DNA-dependent protein kinase catalytic subunit (PRKDC),
Protein kinase C delta type (PRKCD),
Mitogen-activated protein kinase 14 (MAPK14),
Dual specificity tyrosine-phosphorylation-regulated kinase 2 (DYRK2),
Homeodomain-interacting protein kinase 2 (HIPK2).
T55 : MAPK_group,
Mitogen-activated protein kinase 1 (MAPK1),
G protein-coupled receptor kinase 5 (GRK5),
Transcription initiation factor TFIID subunit 1 (TAF1).
Additional Information
Affinity : S46-T55: 3.175 µM, pS46-T55: 0.518 µM, S46-pT55: 0.457 µM, pS46-pT55: 0.097 µM
Structural information: 2GS0
References
(1) Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.
Di Lello et al. Mol. Cell (2006)
See also
Other switches involving participants
Cellular tumor antigen p53 (TP53) - 10 more (view)
